| 松材线虫PCR快速检测方法研究 |
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| 引用本文： | 贺水山,闻伟刚,杨兰英,徐瑛,顾建峰.松材线虫PCR快速检测方法研究[J].植物检疫,2002,16(6):321-324. |
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| 作者姓名： | 贺水山 闻伟刚 杨兰英 徐瑛 顾建峰 |
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| 作者单位： | 宁波出入境检验检疫局,315012 |
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| 摘 要： | 松材线虫是许多国家的检疫性有害生物 ,其形态与拟松材线虫非常相似。为克服传统的形态学鉴定的不足 ,本研究依据松材线虫rDNA基因部分序列 ,通过单个碱基的故意错配 ,设计了一对特异性引物 ,运用PCR方法 ,最终实现了对单条松材线虫的快速检测。该检测方法具有很好的实用性和稳定性
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| 关 键 词： | 松材线虫 PCR 特异性引物 检测 实用性 |
| 修稿时间： | 2002年6月26日 |
The quick detection of Bursaphelenchus xylophilus by PCR |
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| He Shuishan,Wen Weigang,Yang lanying,Xu Ying,Gu Jianfeng.The quick detection of Bursaphelenchus xylophilus by PCR[J].Plant Quarantine,2002,16(6):321-324. |
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| Authors: | He Shuishan Wen Weigang Yang lanying Xu Ying Gu Jianfeng |
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| Abstract: | Bursaphelenchus xylophilus is a quarantine organism in many countries,it is very similar to B mucroratus in morphology To overcome the limitation of the traditional morphological identification of B xylophilus from B mucroratus,we developed the PCR method with using two special primers,which depended on the rDNA gene of B xylophilus by changing a base intentionally This method is very practical and stable in quick detection of individual B xylophilus sample for quarantine department |
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| Keywords: | Bursaphelenchus xylophilus PCR special primers detection practical |
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