贵州省甘蔗审定品种及区试材料的SSR-CE/FD分析

旨在保护贵州育种单位知识产权,指导当地甘蔗杂交育种亲本组合的制定。采用SSR标记和毛细管电泳/荧光检测技术相结合(SSR-CE/FD)的技术,对12个贵州甘蔗材料进行基因型分析,利用NTsys软件进行数据处理。21对多态性高的SSR引物共获得131条扩增带,多态性条带比例为90.1%,平均每对引物产生6.2个条带。其中11个条带为7个品种(系)中某一品种(系)的特征条带;利用这些特征条带可以迅速将该7个品种(系)的中某一品种(系)与其他材料区分开。引物SMC31CUQ多态性条带比例为100%,且可以将12个甘蔗材料完全区分开,是分辨率最高的引物。UPGMA聚类分析表明12个甘蔗无性系间的遗传相似系数变异范围为0.64~0.92。本研究成功构建了贵州3个审定品种和9个区试材料基于131个SSR条带的指纹图谱,12个甘蔗无性系之间遗传基础较为狭窄,育种工作中应选用遗传背景差异大的甘蔗亲本,从而拓宽贵州甘蔗品种(系)的遗传基础。

SSR-CE/FD Assessment of Guizhou Approved Sugarcane Cultivars and Regional Materials

mbination in local sugarcane cross breeding, SSR-capillary electrophoresis/fluorescence detection (SSR-CE/FD) technology was used to analyze twelve sugarcane genotypes from Guizhou. The SSR data were then calculated using NTsys software. A total of 131 DNA fragments were amplified through PCR by 21 pairs of highly polymorphic SSR primers, of which 90.1% were polymorphic. The number of fragments per primer pair was 6.2. Eleven fragments were genotype-specific fragment of one cultivar (line) from seven cultivars (lines); these genotype-specific fragments could distinguish one cultivar (line) from other six cultivars (lines). The SMC31CUQ primer pair, produced 100% polymorphic alleles, was the highest-resolution primer that could distinguish all the 12 sugarcane genotypes. According to the cluster results of UPGMA program, pairwise similarity coefficients among the 12 molecular identities varied from 0.64 to 0.92. Our study successfully constructed a SSR fingerprint of 131 DNA fragments about 3 cultivars and 9 regional materials in Guizhou Province. The twelve sugarcane genotypes have a narrow genetic base, indicating parental materials with big differences in genetic background should be employed in sugarcane breeding programs in order to broaden the genetic basis among sugarcane cultivars (clones) in Guizhou Province.