| 聚丙烯酰胺凝胶电泳技术的改进及其在cDNA-AFLP分析中的应用 |
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| 引用本文： | 曹蕾,徐波,朱万里,张国华,黄木坤,应辰哲,何水林. 聚丙烯酰胺凝胶电泳技术的改进及其在cDNA-AFLP分析中的应用[J]. 福建农林大学学报(自然科学版), 2007, 36(4): 389-391 |
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| 作者姓名： | 曹蕾 徐波 朱万里 张国华 黄木坤 应辰哲 何水林 |
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| 作者单位： | 1. 福建农林大学生命科学学院,福建,福州,350002;福建漳州农业学校,福建,漳州,363000
2. 福建农林大学生命科学学院,福建,福州,350002 |
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| 摘 要： | 利用电解质梯度变性聚丙烯酰胺凝胶电泳系统对稻纵卷叶螟取食的水稻叶片进行cDNA-AFLP分析,结果表明产生的cDNA条带比普通变性聚丙烯酰胺凝胶电泳系统大约增加30%,解决了传统的聚丙烯酰胺凝胶电泳在同一块胶板中大片段DNA条带堆积而小片段DNA条带间距过大,且读带数量有限的问题..
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| 关 键 词： | 电解质梯度 聚丙烯酰胺凝胶 电泳 cDNA-AFLP |
| 文章编号： | 1671-5470（2007）04-0389-03 |
| 修稿时间： | 2007-09-25 |
Improvement of the technique of polyacrylamide gel electrophoresis and its application in cDNA-AFLP analysis |
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| CAO Lei,XU Bo,ZHU Wan-li,ZHANG Guo-hua,HUANG Mu-kun,YIN Chen-zhe,HE Shui-lin. Improvement of the technique of polyacrylamide gel electrophoresis and its application in cDNA-AFLP analysis[J]. Journal of Fujian Agricultural and Forestry University, 2007, 36(4): 389-391 |
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| Authors: | CAO Lei XU Bo ZHU Wan-li ZHANG Guo-hua HUANG Mu-kun YIN Chen-zhe HE Shui-lin |
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| Affiliation: | 1. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Zhangzhou Agriculture Technology School, Zhangzhou, Fujian 363000, China |
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| Abstract: | The improved system of polyacrylamide gel clectrophoresis was used in the cDNA-AFLP analysis of rice leaf, and the result showed that the number of distinguishable bands in the polyacrylamidc gel increased by 30%. It resolved the problem of big DNA fragrncnt stacking, big space between small fragments, and few band number in a gel in cDNA-AFLP analysis. |
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| Keywords: | electrolyte gradient polyacrylamidc gel clcctrophoresis cDNA-AFLP |
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