| 几种有机酸和氨基酸对乙醇酸氧化酶活性的影响 |
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| 引用本文： | 徐凤彩,谭锦华,李明启.几种有机酸和氨基酸对乙醇酸氧化酶活性的影响[J].华南农业大学学报,1988(3). |
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| 作者姓名： | 徐凤彩 谭锦华 李明启 |
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| 作者单位： | 华南农业大学光合作用研究室
(徐凤彩，谭锦华)，华南农业大学光合作用研究室(李明启) |
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| 摘 要： | 本文研究苹果酸、甘油酸、丝氨酸、谷氨酸对菜心(Brassica parachinensis Bailey)叶片乙醇酸氧化酶活性的影响。结果表明,苹果酸、丝氨酸和谷氨酸均对乙醇酸氧化酶起竞争性抑制作用,其中以苹果酸和丝氨酸的抑制作用较强而谷氨酸的抑制作用较弱;测定菜心乙醇酸氧化酶对乙醇酸的Km值为0.36m mol/L,丝氨酸、苹果酸、谷氨酸对乙醇酸氧化酶的Ki值分别为5.5,7.0,13.5m mol/L,甘油酸对乙醇酸氧化酶活性没有明显的影响,讨论了上述几种有机酸和氨基酸在乙醇酸途径代谢调节中的作用。
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| 关 键 词： | 苹果酸 甘油酸 丝氨酸 谷氨酸 乙醇酸氧化酶 |
EFFECTS OF SOME ORGANIC AND AMINO ACIDS ON THE ACTIVITY OF GLYCOLATE OXIDASE FROM LEAVESOFBrassica parachinensis Bailey |
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| Xu Fengcai Tan Jinhua Li Mingqi.EFFECTS OF SOME ORGANIC AND AMINO ACIDS ON THE ACTIVITY OF GLYCOLATE OXIDASE FROM LEAVESOFBrassica parachinensis Bailey[J].Journal of South China Agricultural University,1988(3). |
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| Authors: | Xu Fengcai Tan Jinhua Li Mingqi |
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| Institution: | Laboratory of photosynthesis Resareh |
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| Abstract: | In this communieation we report the effects of malic and glyceric acids, serine, and glutamic acid on the activity of glycolate oxidase from the leaves of Brassica parachinensis Bailey. It was fonnd that malic acid, serine, and glutamic acia inhibited competitively the activity of glycolate oxidase;the Ki values for serine, malic acid, and glutamic acid were 5.5,7.0,and 13.5 m mol/L respectively,while the km (glycolate) of glycolaie oxidase was 0.36m mol/L determined by the Li-neweaver-Burk plots. Glyceric acid showed no marked effect on the activity of this enzyme. The significance of the regulation of glycolate oxidase by these acids in the photorespiratory glycolate pathway is discussed. |
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| Keywords: | malate glycerate serine glutamate glycolate oxidase |
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