珍稀中药材白芨组培快繁体系的建立

为建立珍稀中药材白芨的组培快繁体系,以成熟种子为外植体,筛选适于萌发生长的蔗糖、外源激素(6-BA、IBA、IAA和NAA)和活性炭的浓度、光照时间以及炼苗基质配比。结果发现：在MS+4 g/L琼脂+20 g/L蔗糖+光照(200 lx) 24 h/d的基础上,培养7天后种子均可发芽;添加1.5 mg/L 6-BA+0.5 mg/L NAA+1.0 g/L活性炭,叶片数达5枚;添加1.5 mg/L IBA+1.0 g/L活性炭,根数和根长分别达到3条和4 cm;蛭石:泥炭=1:2的基质配比下,炼苗成活率达100%。该研究结果可为白芨的大规模种植、相关药物生产以及野生资源保护等方面提供理论基础与技术支撑。

Establishment of Tissue Culture and Rapid Propagation System for Rare Medicinal Bletilla striata

To establish the system of tissue culture for Bletilla striata, an experiment was conducted to screen the concentrations of sucrose, exogenous hormones (6-BA, IBA, IAA and NAA) and activated carbon (AC), light duration and hardening-seedling matrix for seed germination and growth taking mature seeds as original explants. The results showed that the seeds germinated after 7 d in the medium MS+4 g/L agar+20 g/L sucrose+ light (200 lx) 24 h/d; the number of cotyledon reached 5 with supplemental 1.5 mg/L 6-BA+0.5 mg/L NAA+1.0 g/L AC; the root number and length respectively reached 3 and 4 cm with supplemental 1.5 mg/L IBA+1.0 g/L AC; and the seedling survival rate reached 100% under the matrix vermiculite: peat (1:2). These findings would provide a theoretical basis and technical support for large-scale cultivation, pharmaceutical production and wild resource protection of Bletilla striata.