热休克蛋白增加运动发酵单胞菌乙醇产量的研究 |
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引用本文: | 李海燕,王茜,苏志国,熊平,汪浩勇. 热休克蛋白增加运动发酵单胞菌乙醇产量的研究[J]. 现代农业科学, 2009, 0(4): 33-36,77 |
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作者姓名: | 李海燕 王茜 苏志国 熊平 汪浩勇 |
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作者单位: | [1]湖北工业大学湖北省工业微生物重点实验室,湖北武汉430068 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 |
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基金项目: | 资助项目:湖北省优秀中青年团队计划项目T200705构建乙醇高温发酵基因工程菌 |
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摘 要: | 目的:对含有Hsp基因的代谢工程运动发酵单胞菌进行热适应处理,用于提高其乙醇产量和对高糖的耐受性。方法:用含有Hsp基因的多顺反子质粒转化运动发酵单胞菌,经过多天37℃热适应后,在玉米水解液中测定该多顺反子对运动发酵单胞菌发酵产生的影响。结果:在37℃发酵条件下,在糖浓度为28%的玉米水解液中,与对照组相比,经过热适应的含Hsp基因的代谢工程菌的乙醇产量显著提高。结论:Hsp基因能够显著提高运动发酵单胞菌的乙醇产量。
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关 键 词: | 逆境适应 运动发酵单胞菌 Hsp 高糖 乙醇产量 |
Adaptation Mediated by Hsp can Improve the Resistance to Adversity and Ethanol Production of Zymomonas mobilis |
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Affiliation: | LI Hai-yan ,WANG Xi, SU Zhi-Guo ,XIONG Ping, WANG Hao-yong (1. Huhei University of Technology, Wuhan,430068;2. Institute of Process Engineering Chinese Academy of Sciences,Beijing, 100190, China) |
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Abstract: | Objective: Hsp was used to mediated the adversity adaptation of Zymomonas mobilis to increase it's resistance to the adversity and ethanol production. Method: Zymomonas mobilis was transformed by plasmid containing Hsp gene, then incubated at 37℃ for adversity adaptation. After days of treatment, the performance of ethanol production of the adapted strain was analyzed in corn hydrolysis media. Result: After adaptation, the production of ethanol by the Zymomonas mobilis with Hsp was 1.55 times more than it's wild type counterpart in corn hydrolysis with sugar concentration as 28% at 37℃.Conclusion: Hsp gene can significantly increase ethanol production of Zymomonas mobilis at high temperature and high sugar concentration. |
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Keywords: | adversity adaptation Zymomonas mobilis Hsp high sugar concentration ethanol production |
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