| 陆地棉锌指蛋白(GZFP)的原核表达 |
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| 引用本文： | 常平安,杨召军,温镭,魏荣.陆地棉锌指蛋白(GZFP)的原核表达[J].西南农业大学学报,2007,29(3):130-133. |
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| 作者姓名： | 常平安 杨召军 温镭 魏荣 |
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| 作者单位： | 重庆邮电大学生物信息学院,重庆400065 |
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| 基金项目： | 国家自然科学基金资助项目（30440032）;重庆邮电大学博士启动基金资助项目（A2005-13） |
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| 摘 要： | 采用大肠杆菌表达体系来获得陆地棉锌指蛋白（GZFP）的融合蛋白．以pMD18-GZFP质粒为模板，用PCR方法扩增获得GZFP基因的编码区序列，将其克隆到表达载体pET28b（＋）中，转化宿主菌BL21（DE3），成功地构建了陆地棉GZFP基因原核表达载体pET—GZFP、经IPTG诱导、SDS-PAGE电泳分析和蛋白质杂交检测表明，陆地棉GZFP以融合蛋白的形式在大肠杆菌中得到大量表达．
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| 关 键 词： | 锌指蛋白 原核表达 陆地棉 |
| 文章编号： | 1000-2642（2007）03-0130-04 |
| 修稿时间： | 2006-09-12 |
Prokaryotic Expression of A Novel Zinc Finger Protein from Upland Cotton (Gossypium hirsutum L.) |
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| CHANG Ping-an, YANG Zhao-jun, WEN Lei, WEI Rong.Prokaryotic Expression of A Novel Zinc Finger Protein from Upland Cotton (Gossypium hirsutum L.)[J].Journal of Southwest Agricultural University,2007,29(3):130-133. |
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| Authors: | CHANG Ping-an YANG Zhao-jun WEN Lei WEI Rong |
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| Institution: | College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China |
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| Abstract: | In this study,a novel zinc finger protein(GZFP) from upland cotton(Gossypium hirsutum L.)was expressed by the E.coli expression system.The GZFP gene was amplified by PCR with pMD18-GZFP plasmid as the template,and was then cloned into the pET-28b(+) to construct pET-GZFP recombinant plasmid,which was transformed into E.coli BL21(DE3).The recombinant GZFP was induced and expressed by IPTG.SDS-PAGE analysis and Western blotting showed that the target protein was expressed at a high level in E.coli BL21(DE3). |
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| Keywords: | zinc finger protein prokaryotic expression Gossypiurn hirsutum L |
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