| 二氧化硅凝胶葡萄糖传感器上羟甲基纤维素酶活性测定 |
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| 引用本文： | 干宁,徐伟民,周雄,王志颖.二氧化硅凝胶葡萄糖传感器上羟甲基纤维素酶活性测定[J].林产化学与工业,2007,27(3):93-97. |
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| 作者姓名： | 干宁 徐伟民 周雄 王志颖 |
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| 作者单位： | 1. 宁波大学,宁波市新型功能材料及其制备科学国家重点实验室培育基地,浙江,宁波,315211;南京大学,生命科学学院,江苏,南京,210008
2. 宁波大学,宁波市新型功能材料及其制备科学国家重点实验室培育基地,浙江,宁波,315211 |
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| 基金项目： | 中国博士后科学基金，浙江省科技攻关项目，浙江省教育厅资助项目，浙江省宁波市自然科学基金 |
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| 摘 要： | 提出了用SiO2凝胶包埋葡萄糖氧化酶(GOD)电化学传感器(GOD/SiO2)测定羟甲基纤维素酶(CMCE)活性的方法,即基于电极对纤维素水解后生成葡萄糖的催化氧化.考察了各种实验条件对CMCE酶活性测定的影响.实验表明,GOD/SiO2电极检测电位为 0.57 V(以饱和甘汞电极为参比电极), 稳态响应电流与纤维素酶活性在5～400 U/L 之间有线性关系,该方法可用于微量羟甲基酶活性测定.
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| 关 键 词： | SiO2凝胶 葡萄糖氧化酶 电化学传感器 纤维素酶 酶活 |
| 文章编号： | 0253-2417（2007）03-0093-05 |
| 修稿时间： | 2006-02-14 |
Determination of Carboxymethyl Cellulase Activity by Glucose Sensor Based on SiO2 Gel Membrane |
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| GAN Ning,XU Wei-min,ZHOU Xiong,WANG Zhi-ying.Determination of Carboxymethyl Cellulase Activity by Glucose Sensor Based on SiO2 Gel Membrane[J].Chemistry & Industry of Forest Products,2007,27(3):93-97. |
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| Authors: | GAN Ning XU Wei-min ZHOU Xiong WANG Zhi-ying |
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| Institution: | 1. The State Key Labormory base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, China; 2. Biochemistry Department of Faculty of Life Science, Nanjing Univerisity, Nanjing 210008, China |
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| Abstract: | A new biosensor for the assay of carboxymethyl cellulase(CMCE) activity was prepared by modifying a glucose oxidase (GOD) on SiO2 gel film coated on glassy carbon electrode. The amperometric determination was based on electrochemically detected glucose produced by enzymatic reaction of sodium carboxymethyl cellulose(CMC-Na) under the catalysis of CMCE. The modified electrode can sensitively detect CMCE at 0.57 V vs. SCE with a linear response in the range of 5 U/L to 400 U/L under an optimum condition and can be used as a promising method for determination of CMCE activity in micro-analysis. |
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| Keywords: | SiO2 gel glucose oxidase electrochemical sensor cellulase enzyme activity |
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