IGF1对不同培养代数梅花鹿鹿茸间充质层细胞增殖的影响

【研究目的】通过细胞培养技术，探讨胰岛素样生长因子1（IGF1）对体外培养不同代数的梅花鹿鹿茸间充质层细胞增殖的影响。【方法】分离、培养生长90d的梅花鹿鹿茸间充质层细胞，将传代培养第2代、5代、8代细胞经含有不同浓度的IGF1（0，1，3，和10 nM）作用，培养24 h后用3H-胸腺嘧啶核苷掺入测定法检测蛋白合成放射性含量。【结果】不同浓度IGF1处理组的蛋白合成放射性含量的平均值和最高值都是离体培养2代的细胞最高（分别为32146和35973 DPM/mg）；培养5代的细胞（分别为2781和3206 DPM/mg）已明显下降；第8代时（分别为489和736 DPM/mg）又进一步下降，并且与对照组无显著的差异。【结论】不同培养时期的梅花鹿鹿茸间充质层细胞对IGF1刺激的敏感度不同，取材于生长90天鹿茸的间充质层细胞在离体培养8代后已失去了对IGF1刺激的敏感性。

Effects of Insulin-Like Growth Factor 1 on the Proliferation of Sika Deer Antler Mesenchymal Layer Cells at Different Generation

To investigate the effects of insulin-like growth factor 1 (IGF1) on the proliferation of sika deer antler mesenchymal layer cells from different passages in vitro. The mesenchymal layer cells were isolated from sika deer antlers of growing 90 day and cultured. The second, fifth and eighth population cells were incubated with 0, 1, 3, and 10 nM IGF1. Thymidine incorporation assay was applied after 24 hours of culture respectively to determine the radioactivity content of protein synthesis. The average and m...