| 黄瓜染色体片段导入系的构建与遗传评价 |
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| 引用本文： | 史建磊,娄群峰,钱春桃,万红建,周晓慧,陈劲枫. 黄瓜染色体片段导入系的构建与遗传评价[J]. 南京农业大学学报, 2011, 34(1) |
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| 作者姓名： | 史建磊 娄群峰 钱春桃 万红建 周晓慧 陈劲枫 |
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| 作者单位： | 南京农业大学园艺学院/作物遗传与种质创新国家重点实验室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金项目(30671419,30700541),国家自然科学基金重点项目(30830079); 国家高技术研究发展计划专项经费(2008AA10Z150,2006AA10Z1A8,2006AA100108); 作物遗传与种质创新国家重点实验室开放基金项目(ZW2007010) |
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| 摘 要： | 以综合性状优良的栽培黄瓜(Cucumis sativus L.,2n=14)北京截头为受体亲本,以携带有多种抗性性状的黄瓜珍稀野生种酸黄瓜(Cucumis hystrix Chakr.,2n=24)为供体亲本,在本实验室前期获得种间杂交异源四倍体的基础上,通过多代回交和自交,结合SSR标记辅助选择构建了以北京截头为遗传背景的黄瓜野生种染色体片段导入系,并对其导入片段的数目、分布、大小和覆盖率等进行了评价。结果表明:该导入系群体携带的38个供体片段不均匀地分布于黄瓜7条染色体上,其长度介于1.1~14.9 cM,平均长度为5.3 cM,总长度为201.9 cM,在黄瓜基因组上的覆盖率为23.5%。
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| 关 键 词： | 黄瓜 SSR标记 染色体片段导入系 评价 |
Construction and genetic evaluation of chromosome segment introgression lines in cucumber |
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| SHI Jian-lei,LOU Qun-feng,QIAN Chun-tao,WAN Hong-jian,ZHOU Xiao-hui,CHEN Jin-feng. Construction and genetic evaluation of chromosome segment introgression lines in cucumber[J]. Journal of Nanjing Agricultural University, 2011, 34(1) |
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| Authors: | SHI Jian-lei LOU Qun-feng QIAN Chun-tao WAN Hong-jian ZHOU Xiao-hui CHEN Jin-feng |
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| Affiliation: | SHI Jian-lei,LOU Qun-feng,QIAN Chun-tao,WAN Hong-jian,ZHOU Xiao-hui,CHEN Jin-feng(College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | The cultivated cucumber(Cucumis sativus L.,2n=14)Beijingjietou with comprehensive good traits and precious rare wild sour cucumber(Cucumis hystrix Chakr.,2n=24)carrying a variety of resistance traits were used as receptor parent and donor parent respectively in the present study.Chromosome segment introgression lines(CSILs)were developed on the basis of interspecific allotetraploid by multiple generational backcrossing and selfing,combined with SSR marker-assisted selection(MAS),with the genetic background ... |
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| Keywords: | cucumber SSR markers chromosome segment introgression lines(CSILs) evaluation |
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