| 8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响 |
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| 引用本文： | 王君敏,胡元亮,张帆,王德云,赵晓娜,张靖,刘静,赵彪,郝丽华.8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响[J].南京农业大学学报,2011,34(1). |
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| 作者姓名： | 王君敏 胡元亮 张帆 王德云 赵晓娜 张靖 刘静 赵彪 郝丽华 |
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| 作者单位： | 1. 南京农业大学中兽医学研究室,江苏南京,210095
2. 中国兽医药品检查所,北京,100081 |
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| 基金项目： | 国家自然科学基金项目(30871887); 国家科技支撑计划项目(2008BADB4B06) |
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| 摘 要： | 用氯磺酸-吡啶法修饰当归多糖(CAPS)和枸杞多糖(LBPS),得到4种硫酸化当归多糖sCAPS0.6、sCAPS1.1、sCAPS1.9、sCAPS2.2和4种硫酸化枸杞多糖sLBPS0.7、sLBPS1.1、sLBPS1.5和sLBPS1.9。分别以CAPS和LBPS为对照,采用MTT法比较了这8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响。结果表明:硫酸化修饰能显著提高当归多糖和枸杞多糖的抗病毒活性,且与硫酸基取代度有一定的相关性。sCAPS2.2、sLBPS1.5、sCAPS1.9和sLBPS1.9的活性较好,可以作为进一步研究的材料。
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| 关 键 词： | 硫酸化修饰 当归多糖 枸杞多糖 新城疫病毒 感染细胞能力 鸡胚成纤维细胞 |
Effects of eight sulfated polysaccharides on infecting chicken embryo fibroblast of Newcastle disease virus |
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| WANG Jun-min,HU Yuan-liang,ZHANG Fan,WANG De-yun,ZHAO Xiao-na,ZHANG Jing,LIU Jing,ZHAO Biao,HAO Li-hua.Effects of eight sulfated polysaccharides on infecting chicken embryo fibroblast of Newcastle disease virus[J].Journal of Nanjing Agricultural University,2011,34(1). |
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| Authors: | WANG Jun-min HU Yuan-liang ZHANG Fan WANG De-yun ZHAO Xiao-na ZHANG Jing LIU Jing ZHAO Biao HAO Li-hua |
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| Institution: | WANG Jun-min1,HU Yuan-liang1,ZHANG Fan1,WANG De-yun1,ZHAO Xiao-na1,ZHANG Jing1,LIU Jing1,ZHAO Biao1,HAO Li-hua2(1.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China,2.China Institute of Veterinary Drug Control,Beijing 100081,China) |
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| Abstract: | Chinese angelica polysaccharide(CAPS)and lycium barbarum polysaccharide(LBPS)were modified by chlrulfonic-pyridine method to obtain four sulfated CAPS,sCAPS0.6,sCAPS1.1,sCAPS1.9 and sCAPS2.2,and four sulfated LBPS,sLBPS0.7,sLBPS1.1,sLBPS1.5 and sLBPS1.9.The effects of eight sulfated polysaccharides on cellular infectivity of Newcastle disease virus(NDV)were compared by MTT method taking the non-modified CAPS and LBPS as controls.The results showed that sulfated modification could significantly enhance the a... |
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| Keywords: | sulfated modification Chinese angelica polysaccharide lycium barbarum polysaccharide Newcastle disease virus cellular infectivity chicken embryo fibroblast |
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