四川境内变电站RTV涂料绝缘子上藻类的分子系统学研究

目的]对四川省境内变电站RTV涂料绝缘子表面藻类的生长情况进行了调研,并对其进行分子系统学分析。方法]选取4个藻类生长比较典型的地点,对4地RTV涂料绝缘子上生长的藻类进行实地取样,用光学显微镜对其形态进行初步鉴定,并进一步使用18S r DNA基因进行系统进化分析。结果]通过显微观察发现,4地优势藻类均为单细胞球状绿藻;系统进化分析发现,雅安藻种属于渡边球藻分支,与Heterochlorella luteoviridis亲缘关系较近,在MP、NJ和BI树中的支持率分别为86%、79%、1.0,将其初步鉴定为Heterochlorella sp.SCYA1;广安藻种属于Elliptochloris,与Elliptochloris subsphaerica的18S r DNA序列碱基没有差异,在MP、NJ和BI树中的支持率分别为97%、99%和1.0,故确认其为Elliptochloris subsphaerica SCGA8藻种;巴中和南充藻种都属于栅藻属,两者亲缘关系很近且序列只有2个碱基差异,MP、NJ和BI树中的支持率比较高,分别为93%、98%和1.0,将其初步鉴定为Scenedesmus sp.SCBZ5和Scenedesmus sp.SCNC7。结论]4地变电站RTV涂料绝缘子上的优势藻种不全是同一种藻,生长于四川省境内RTV涂料绝缘子上的藻类具有多样性。

Molecular Phylogenetic Study of Algaes on RTV Coating Insulators at Substations of Sichuan Province

Objective] The algaes growth on the surface of the insulators coated with RTV (room temperature vulcanized silicone rubber)at Sichuan Province was investigated,the molecular systematics were analyzed.Method] Several typical substations were selected.Algaes collected from the surface of the insulators located in those areas were used to explore species diversity.Those dominant algaes were separately purified by spread plate method.The morphology was identified by optical microscopy and the phylogenetic analysis of four monoclonal algaes were carried out based on 18S rRNA sequences.Result] Those algaes were single-celled globular algaes examined by light microscopy.Phylogenetic analysis indicated that the Ya'an dominant algae was identified as Heterochlorella sp.SCYA1,which was closely related to Heterochlorella luteoviridis.The support rates of the two species were 86％,79％,1.0 in MP,NJ and BI trees,respectively.The 18S rDNA sequence of Guang'an dominant algae was not different from that of Elliptochloris subsphaerica,the support rates of the two species in the MP,NJ and BI trees were 97％,99％ and 1.0,respectively,it was identified as Elliptochloris subsphaerica SCGA8.Both Bazhong and Nanchong dominant algaes belonged to Scenedesmus,which had close genetic relationship,they were identified as Scenedesmus sp.SCBZ5 and Scenedesmus sp.SCNC7.The 18S rDNA sequence of two species only had two base differences,their support rates in the MP,NJ and BI trees were 93％,98％ and 1.0.Conclusion] Results indicate that four dominant algaes are not the same kind,algaes growing on the surface of RTV coated insulators at Sichuan Province have species diversity.