软枣猕猴桃原生质体酶解液配方筛选

[目的]筛选软枣猕猴桃原生质体最佳酶解液配方。[方法]以4种配方酶解液在相同条件下处理等量软枣猕猴桃叶片,比较最后收集的纯化原生质体量,筛选最佳酶解液配方。[结果]配方Ⅲ(1.0%纤维素酶、0.5%离析酶、0.3%果胶酶)和Ⅳ(1.0%纤维素酶、0.5%果胶酶)处理酶解效果要明显优于配方Ⅰ(2.0%纤维素酶、0.5%离析酶)、Ⅱ(2.0%纤维素酶、0.3%果胶酶)。[结论]配方Ⅲ(1.0%纤维素酶、0.5%离析酶、0.3%果胶酶)为软枣猕猴桃原生质体最佳酶解液配方。

Screening of Enzymatic Hydrolysate Formula of Actinidia arguta Protoplast

[Objective] To screen the best enzymatic hydrolysate formula of Actinidia arguta protoplast.[Method] The leaves of A.arguta were treated with four enzymatic hydrolysate formula under the same conditions.The purified protoplast was collected and compared to screen the optimal enzymatic hydrolysate formula.[Result] The amount of protoplast collected by the solution of formula Ⅲ (1.0％ cellulase,0.5％ segregation enzyme and 0.3％ pectinase) and formula Ⅳ (1.0％ cellulase,0.5％ pectinase)were significantly higher than the solution of formula Ⅰ (2.0％ cellulase,0.5％ segregation enzyme) and formula Ⅱ (2.0％ cellulase,0.3％ pectinase).[Conclusion] Formula Ⅲ (1.0％ cellulase,0.5％ segregation enzyme and 0.3％ pectinase) is the best enzymatic hydrolysate formula for A.arguta protoplast.