利用SSR标记技术检测玉米杂交种纯度

对玉米子粒DNA提取、SSR扩增片段检测方法等进行了研究,并以4个利用常规种子贮藏蛋白质电泳技术难以鉴定纯度的玉米杂交种及相应自交系和9对玉米SSR位点引物为材料,分别筛选出适合这些不同杂交种纯度鉴定的SSR位点引物,建立了一套利用SSR标记进行玉米杂交种纯度鉴定的技术规程.该程序包括从粉碎干种子提取DNA和利用非变性聚丙烯酰胺凝胶电泳分离SSR扩增片段,对仪器设备要求较低、简单、快速,易于推广。

Purity Testing Method for Maize Hybrids Using SSR Markers

In this study, (1)two sets of DNA samples were extracted from ground dry seeds and seedlings respectively and compared for their qualities and suitability for PCR amplification; (2)non-denaturing polyacrylamide gel electrophoresis (PAGE) was evaluated for its usefulness in separating maize SSR amplification fragments; (3)9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purities of 4 maize hybrids that lack a reliable laboratory method for purity testing. The results are as follows: (1)DNA samples extracted from ground dry seeds are comparable in quality to those from seedlings and amplified no differently from the later; (2)non-denaturing PAGE can detect a large proportion of variation in SSR allele sizes and thus is useful in hybrid purity testing; (3)Polymorphic SSR loci have been found that could be applied to purity testing of these 4 hybrids. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker.