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雷竹不同栽培类型RAPD分子标记的研究
引用本文:方伟,何祯祥,黄坚钦,史钱均,林新春. 雷竹不同栽培类型RAPD分子标记的研究[J]. 浙江林学院学报, 2001, 18(1): 1-5
作者姓名:方伟  何祯祥  黄坚钦  史钱均  林新春
作者单位:1. 浙江林学院 竹类研究所,浙江 临安 311300
2. 南京林业大学 森林资源与环境学院,江苏 南京 210037
摘    要:对雷竹的19个栽培类型及2个近缘种进行了RAPD分析。从4组80个引物中筛选出10个引物,产生随机扩增位点66个,其中多态性位点54个(占81.8%)。利用POPGEN32程序进行聚类分析,结果表明:(1)聚类结果可将雷竹的不同栽培类型及其近缘种区分开来,以前认为是雷竹一栽培型的永嘉雷竹不属于Ph.praecox种系;(2)细叶雷竹与阔叶雷竹的产量高低主要取决于经营管理;(3)RAPD分子标记对竹类植物种以下等进行分类有一定的可靠性。图2表3参8。

关 键 词:雷竹 RAPD分析 分子标记 栽培类型 笋用竹 分类鉴定 产量
文章编号:1000-5692(2001)01-0001-05
修稿时间:2000-11-27

Studies on cultivars ofPhyllostachys pxaecox with RAPD molecular markers
FANG Wei ,HE Zheng xiang ,HUANG Jian qin ,SHI Qian jun ,LIN Xin chun. Studies on cultivars ofPhyllostachys pxaecox with RAPD molecular markers[J]. Journal of Zhejiang Forestry College, 2001, 18(1): 1-5
Authors:FANG Wei   HE Zheng xiang   HUANG Jian qin   SHI Qian jun   LIN Xin chun
Affiliation:FANG Wei 1,HE Zheng xiang 2,HUANG Jian qin 1,SHI Qian jun 1,LIN Xin chun 1
Abstract:With RAPD molecular markers,19 cultivars and 2 affinis species ofPhyllostachys praecox were analysed.Using 10 primers screened from 80 primers,66 loci were amplified,and 54 of these were polymorphic(81.8%).The UPGMA cluster analysis of POPGEN 32 program showed:(1)The cultivars and affinis species of Ph. praecox can be divided by UPGMA analysis,and the Yongjia Lei Bamboo recognized as one of the cultivars of Lei Bamboo before doesnt belong to Ph. praecox.(2)The production of narrow-leaved Lei Bamboo and broad-leaved Lei Bamboo mainly decided by management.(3)The classification on bamboo under species based on RAPD markers is somewhat reliable.
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