槲皮素对过氧化氢诱导的人红细胞过氧化损伤的抑制作用

目的 :观察槲皮素对H2 O2 诱导的人红细胞过氧化损伤的抑制作用。方法 :用健康成人静脉血制成 1%红细胞 (RBC)悬液 ,分为损伤组、槲皮素组 (槲皮素 +H2 O2 )、丹参组 (丹参注射液 +H2 O2 ,作为阳性对照 ) ,孵育后测定RBC溶血度和RBC悬液中丙二醛 (MDA)浓度。结果 :槲皮素组和丹参组的溶血度及MDA的含量均低于损伤组 (P <0 .0 1) ,且与药物浓度有一定的量效关系。同样浓度的槲皮素、丹参对溶血度的影响差异无显著性 (P均 >0 .0 5 ) ,而在降低MDA含量的效果上 ,槲皮素优于丹参 (P均 <0 .0 1)。结论 :槲皮素能抑制H2 O2 引起的人红细胞溶血和红细胞脂质过氧化作用。

Quercetin inhibits hydroperoxide-induced peroxidation of human red blood cells

Objective:To investigate the effect of quercetin on hydroperoxide induced peroxidation of human red blood cells(RBC).Methods:1% RBC suspension prepared from healthy subjects was divided into 3 groups;control group only treated with hydroperoxide,quercetin group with hydroperoxide plus quercetin,and tanshione group with hydroperoxide plus tanshione.Hemolysis and malondialdehyde(MDA)level of RBC were determined.Results:Compared with control group,quercetin or tanshione treatment resulted in a dose dependent decrease of hemolytic degree and MDA content of RBC( P< 0.01).Although there was no significant difference in hemolytic degree between quercetin and tanshione treatment at the same dose( P> 0.05),MDA level was obviously lower in quercetin group than that in tanshione group( P< 0.01) .Conclusion:Quercetin is able to inhibit hydroperoxide induced hemolysis and peroxidation of human RBC.