绿色荧光蛋白转基因小鼠超数排卵研究

目的]为建立稳定、高效的绿色荧光蛋白转基因小鼠超数排卵技术提供依据。方法]将30只5~8周龄的绿色荧光蛋白转基因小鼠随机平均分成3组,分别腹腔注射5、7.5、10 IU的PMSG,48 h后腹腔注射7.5 IU的HCG,比较不同剂量PMSG对绿色荧光蛋白转基因小鼠超数排卵效果的影响。结果]PMSG剂量为7.5 IU处理组获得的胚胎数明显高于PMSG剂量为5、10 IU处理组,且差异显著(P<0.05)。PMSG剂量为5、7.5、10 IU处理组的小鼠见栓率分别为70%、80%、90%,处理间差异不显著(P>0.05)。随着PMSG剂量的加大,小鼠的子宫充血程度和卵巢体积逐渐增大。结论]绿色荧光蛋白转基因小鼠超数排卵技术中所用PMSG的最佳剂量为7.5 IU。

Study on Superovulation of Transgenic Mice with Green Fluorescent Protein

Objective] The study was to provide the basis for establishing superovulation technique with stable and high efficiency for transgenic mice with green fluorescent protein.Method] 30 transgenic mices with green fluorescent protein at 5～8 weeks old were randomly divided into 3 groups,the intraperitoneal injection was made on them with PMSG at 5,7.5 and 10 IU resp.,and then after 48 h,the intraperitoneal injection was made on them with HCG at 7.5 IU,and the effects of different doses of PMSG on superovulation effect of transgenic mice with green fluorescent protein were compared.Result] The embryonic number of treatment group with PMSG at 7.5 IU was higher than that of the treatment groups with PMSG at 5 and 10 IU,and their difference was significant(P<0.05).The rate of seeing vaginal plug of mice in treatment groups with PMSG at 5,7.5 and 10 IU were 70%,80% and 90% resp.,but the differences among treatments were not significant(P>0.05).With the increasing of PMSG dose,the uterus peremia degree and ovarian volume of the mice were increased gradually.Conclusion] In the superovulation technique for transgenic mice with green fluorescent protein,the optimum dose of PM optimum dose SG was 7.5 IU.