一种适用于PCR的环境友好型植物DNA提取方法(英文)

改进DNA提取方法,利用含2%CTAB、1%SDS、2%PVP、100mmol/LTris-Cl(pH8.0)、20mmol/LEDTA(pH8.0)和1.4mol/LNaCl的DNA提取缓冲液,提取了不同种属12种植物的DNA,并对其进行了PCR检测.结果显示,所提取的DNA符合PCR试验要求,用真核生物ITS4/ITS5通用引物均能扩增出清晰的条带.该方法无需酚仿抽提,简单、高效、环保,特别适合大规模植物DNA的提取.

Plant DNA extraction for PCR: A simple and environment-friendly approach

A simple and environment-friendly procedure for isolating high-quality plant DNA was presented in this paper. The extraction buffer contained 2%CTAB, 1% SDS, 2%PVP, 100 mmol/L Tris-Cl (pH8.0), 20 mmol/L EDTA (pH8.0) and 1.4 mol/L NaCl. In this way, DNA of 12 various plants was extracted and successfully used for PCR amplification by ITS4/ITS5 eukaryotic universal primers. This DNA extract method left out phenol-chloroform extraction, which was safe and time-effective and made it attractive in large-scale DNA extraction.