甘草、黄连等五味中药对体外抗菌作用的研究

为验证清热解毒类中药体外联合抗菌协同指数(SI)判定标准的适用性。采用微管-平板法测定甘草(Radix glycyrrhiza)、黄连(Rhizoma coptidis)、金银花(Flos lonicerae japonicae)、黄柏(Cortex phellodendri chinensis)和秦皮(Cortex fraxini)的水提物和乙醇提取物对猪链球菌Ⅱ型的抗菌活性，用棋盘微量肉汤稀释法测定这五味药不同提取物两两配伍的协同指数，然后将其组方，分别水煎煮和醇提取，并测定其对猪链球菌Ⅱ型的最小抑菌浓度(MIC)。结果表明，黄连和秦皮配伍SI为0.5，甘草和秦皮、甘草和金银花的SI都为0.75，甘草和黄连组方后醇提物对猪链球菌Ⅱ型的MIC为7.81 mg/mL，甘草和秦皮组方后醇提物对猪链球菌Ⅱ型的MIC为1.95 mg/mL，黄连和黄柏组方后的水提物对猪链球菌Ⅱ型的MIC为0.98 mg/mL，黄连和秦皮组方后水提物对猪链球菌Ⅱ型的MIC为1.95 mg/mL。由此表明，黄连醇提物和秦皮水提物的体外联合的协同作用明显，黄连和黄柏药对的水提物对猪链球菌Ⅱ型的抑制作用较好。

The antibacterial action of radix glycyrrhiza, rhizomacoptidis etc five traditional Chinese herbs in vitro

In order to validate the applicability of synergistic index(SI) decision criteria on mutual compatibility antibacterial activity of clearing heat and removing toxicity traditional Chinese medicine in vitro,antibacterial activity to streptococcus was detected by the microtube-flat method on water extract and ethanol extract from the Radix glycyrrhiza,Rhizoma coptidis,Flos lonicerae japonicae,Cortex phellodendri chinensis and Cortex fraxini.SI was detected by the board trace broth dilution.SI was calculated by the formula.And then two Chinese herbs were matched into compound.The compound was extracted by water and ethanol solution.The minimum antibacterial concentration(MIC) of the extraction to streptococcus was determined.The results showed that SI of Rhizoma coptidis and Cortex fraxini compatibility was 0.5,while SI of Radix glycyrrhiza and Cortex fraxini,Radix glycyrrhiza and Flos lonicerae japonicae was both 0.75.MIC of ethanol-extraction to streptococcus,suis Ⅱ was 7.81 mg/mL.MIC of Radix glycyrrhiza and Rhizoma coptidis,Radix glycyrrhiza and Cortex fraxini ethanol-extraction was both 1.95 mg/mL.MIC of Rhizoma coptidis and Cortex phellodendri chinensis water-extract was 0.98 mg/mL.MIC of Coptis chinensis franch and Cortex fraxini water was 1.95 mg/mL.This showed that the compatibility on Rhizoma coptidis ethanol-extraction content and Cortex fraxini water extraction was present synergy obviously in vitro,the antibacterial activity ofwater-extraction on Rhizoma coptidis and Cortex phellodendri chinensis to streptococcus suis Ⅱ was the strongest.