| PCR-SSCP用于针叶树种遗传分析的可行性 |
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| 引用本文： | 卢孟柱,王晓茹,Alfred E. Szmidt.PCR-SSCP用于针叶树种遗传分析的可行性[J].林业科学研究,2000,13(4):349-354. |
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| 作者姓名： | 卢孟柱 王晓茹 Alfred E. Szmidt |
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| 作者单位： | 1. 中国林业科学研究院,林业研究所,北京,100091
2. 瑞典农业大学,森林遗传与生理系,瑞典于默奥,90183 |
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| 基金项目： | 中国—瑞典科技合作项目 |
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| 摘 要： | 利用松树单倍体胚乳，双倍体的针叶材料，扩增了叶绿体基因组和核基因组的５个ＤＮＡ片段，研究了ＳＳＣＰ这一分析方法的可靠性。结果表明ＳＳＣＰ分析方法具有坚实的分子基础、较高的分辨率和试验重复性。对ＳＳＣＰ谱带在杂交子代和单倍体胚乳的分离分析，证明了ＳＳＣＰ还具有良好的遗传稳定性。
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| 关 键 词： | PCR－SSCP 针叶树 遗传分析 多态型 |
| 收稿时间： | 3/2/2000 12:00:00 AM |
Application of PCR SSCP in Genetic Analysis of Conifers |
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| LU Meng-zhu,WANG Xiao-ru and Alfred E. Szmidt.Application of PCR SSCP in Genetic Analysis of Conifers[J].Forest Research,2000,13(4):349-354. |
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| Authors: | LU Meng-zhu WANG Xiao-ru and Alfred E Szmidt |
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| Institution: | Research Institute of Forestry, CAF, Beijing 100091, China;Department of Forest Genetics, SLU, Umea 90183, Sweden;Department of Forest Genetics, SLU, Umea 90183, Sweden |
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| Abstract: | Five DNA fragments located in chloroplast and nuclear genome were amplified from DNA extracted from haploid endosperm and diploid needle of Pinus ,and were further used in the characterization of the PCR SSCP technique.The results show that the technique has solid molecular basis,high reproducibility and sensitivity.The analysis of the SSCP bands using controlled cross and haploid endosperm has confirmed their Mendelian inheritance.Therefore PCR SSCP could find many uses in phylogenetic and population genetic analysis in conifers. |
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| Keywords: | PCR SSCP conifer genetic analysis polymorphic |
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