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| 采用花粉管通道法将蛋白激酶基因导入玉米自交系的研究 | |
| 引用本文： | 石薇,黄丛林,张秀海,吴忠义,杨德光. 采用花粉管通道法将蛋白激酶基因导入玉米自交系的研究[J]. 华北农学报, 2011, 26(4): 46-49 |
| 作者姓名： | 石薇 黄丛林 张秀海 吴忠义 杨德光 |
| 作者单位： | 1. 北京市农林科学院北京农业生物技术研究中心,北京100097;东北农业大学农学院,黑龙江哈尔滨150030 2. 北京市农林科学院北京农业生物技术研究中心,北京,100097 3. 东北农业大学农学院,黑龙江哈尔滨,150030 |
| 基金项目： | 国家转基因重大专项重点课题(2009ZX08003-009B); 北京市科委项目(Z090605006009014;Z09090501040902); 北京市农林科学院科研能力创新项目 |
| 摘 要： | 通过花粉管通道法将ZmPtil,ZmPtil-1,ZmCIPK2三种蛋白激酶基因植物表达载体分别导入玉米自交系吉444,经草丁膦筛选后得40株抗性植株,通过PCR检测其中28株为PCR阳性植株,平均转化率为1.33％,最后收获11株转基因植株.干旱条件下通过对转基因T1植株的单株籽粒产量、千粒重两个指标进行差异显著性和... |
| 关 键 词： | 蛋白激酶 花粉管通道法 转基因玉米 抗旱性 |
Studies on Transformation of Maize Inbred Line with Protein Kinase Genes by Pollen-tube-pathway |
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| SHI Wei,HUANG Cong-lin,ZHANG Xiu-hai,WU Zhong-yi,YANG De-guang. Studies on Transformation of Maize Inbred Line with Protein Kinase Genes by Pollen-tube-pathway[J]. Acta Agriculturae Boreali-Sinica, 2011, 26(4): 46-49 | |
| Authors: | SHI Wei HUANG Cong-lin ZHANG Xiu-hai WU Zhong-yi YANG De-guang |
| Affiliation: | SHI Wei 1,2,HUANG Cong-lin1,ZHANG Xiu-hai1,WU Zhong-yi1,YANG De-guang2 (1.Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China,2.College of Agronomy,Northeast Agricultural University,Harbin 150030,China) |
| Abstract: | Three plant expression vectors harboring protein kinase genes of ZmPti1,ZmPti1-1,or ZmCIPK2 were transferred into maize inbred line Ji 444 separately by pollen-tube-pathway,and 40 glufosinate-tolerant plants were obtained through herbicide screening,then 28 of them were confirmed positive by PCR analysis.The average transformation rate was 1.33%,and 11 transgenic lines were harvesting T1 seeds.Control plants along with T1 transgenic plants were cultivated under drought conditions,and the yield of each plant... |
| Keywords: | Protein kinase Pollen-tube-pathway Transgenic maize Drought tolerance |
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