| 苏云金芽孢杆菌SHJ-5菌株杀虫晶体蛋白基因分析 |
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| 引用本文： | 杜立新,董明,王容燕,马铭泽,王金耀,曹伟平,宋健,冯书亮.苏云金芽孢杆菌SHJ-5菌株杀虫晶体蛋白基因分析[J].华北农学报,2011,26(4):194-197. |
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| 作者姓名： | 杜立新 董明 王容燕 马铭泽 王金耀 曹伟平 宋健 冯书亮 |
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| 作者单位： | 1. 河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000
2. 河北省农林科学院,河北石家庄,050051 |
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| 基金项目： | 国家“863”计划项目(2011AA10A203); 国家科技支撑项目(2006BAD08A02);国家科技支撑项目(2008BADA5B07) |
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| 摘 要： | 苏云金芽孢杆菌SHJ-5菌株是从黑龙江采集的土样中分离获得的新菌株,对其伴孢晶体形状、基因型、蛋白表达以及杀虫活性进行了系统研究.菌株SHJ-5产生的伴孢晶体形状为钝菱形；利用PCR-RFLP技术对该菌株的基因型进行鉴定,结果表明,该菌株含有cry1Aa、cry1Ea、cry1Be、cry1Ah、cry1Ai、cry2...
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| 关 键 词： | 苏云金芽孢杆菌 PCR-RFLP 克隆 杀虫活性 |
Identification of cry Gene from a Novel Bacillus thuringiensis Isolate SHJ-5 |
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| DU Li-xin,DONG Ming,WANG Rong-yan,MA Ming-ze,WANG Jin-yao,CAO Wei-ping,SONG Jian,FENG Shu-liang.Identification of cry Gene from a Novel Bacillus thuringiensis Isolate SHJ-5[J].Acta Agriculturae Boreali-Sinica,2011,26(4):194-197. |
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| Authors: | DU Li-xin DONG Ming WANG Rong-yan MA Ming-ze WANG Jin-yao CAO Wei-ping SONG Jian FENG Shu-liang |
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| Institution: | DU Li-xin1,DONG Ming1,WANG Rong-yan1,MA Ming-ze2,WANG Jin-yao1,CAO Wei-ping,1,SONG Jian1,FENG Shu-liang1(1.Institute of Plant Protection,Hebei Academy of Agriculture and Forestry Sciences,IPM Centre of Hebei Province,Baoding 071000,China,2.Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China) |
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| Abstract: | The novel Bacillus thuringiensis SHJ-5 was isolated from the soil in Heilongjiang province.This paper was a study on the strain SHJ-5,including that the shape of the crystal,the cry-type,protein expression and insecticidal activity.It produced blunt diamond-shaped crystals.It was identified that strain SHJ-5 contained cry1Aa,cry1Ea,cry1Be,cry1Ah,cry1Ai,cry2Ab,cry1I and vip3A gene by PCR-RFLP.SDS-PAGE analysis indicated that the strain produced a 130-150 kDa protein and a 60 kDa protein.Bioassay showed that ... |
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| Keywords: | Bacillus thuringiensis PCR-RFLP Clone Insecticidal activity |
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