| 环介导等温扩增技术检测含有CaMV35S的转基因玉米 |
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| 引用本文： | 陈金松,黄丛林,张秀海,吴忠义.环介导等温扩增技术检测含有CaMV35S的转基因玉米[J].华北农学报,2011,26(4):8-14. |
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| 作者姓名： | 陈金松 黄丛林 张秀海 吴忠义 |
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| 作者单位： | 1. 北京市农林科学院北京农业生物技术研究中心,北京100097;首都师范大学生命科学学院,北京100048
2. 北京市农林科学院北京农业生物技术研究中心,北京,100097 |
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| 基金项目： | 国家转基因专项资助项目(2009ZX08003-009B); 北京市科委项目(KJCX201102003) |
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| 摘 要： | DNA环介导等温扩增技术是一种特异、灵敏、快速的新型基因检测技术.针对玉米表达载体的花椰菜花叶病毒35 S启动子(CaMV35S)的6个区域设计4种特异引物,对LAMP反应的MgSO4、dNTPs、Betaine、内引物、外引物各个成分进行了优化,此外还对LAMP和PCR两种不同方法的特异性进行了比较.建立转基因玉米花...
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| 关 键 词： | 转基因玉米 环介导等温扩增技术(LAMP) 检测方法 |
Loop-mediated Isothermal Amplification for the Detection of CaMV35S Promoter in Genetically Modified Maize |
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| CHEN Jin-song,HUANG Cong-lin,ZHANG Xiu-hai,WU Zhong-yi.Loop-mediated Isothermal Amplification for the Detection of CaMV35S Promoter in Genetically Modified Maize[J].Acta Agriculturae Boreali-Sinica,2011,26(4):8-14. |
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| Authors: | CHEN Jin-song HUANG Cong-lin ZHANG Xiu-hai WU Zhong-yi |
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| Institution: | CHEN Jin-song 1,2,HUANG Cong-lin1,ZHANG Xiu-hai1,WU Zhong-yi1 (1.Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China,2.College of Life Science,Capital Normal University,Beijing 100048,China) |
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| Abstract: | The objective is to develop a loop-mediated isothermal amplification method for detection of CaMV-35S promoter in Genetically Modified maize.Loop-mediated isothermal amplification(LAMP),a novel nucleic acid amplification method,was developed for the rapid detection of Genetically Modified maize.Cauliflower mosaic virus 35S(CaMV35S) promoter gene was amplified by a set of four primers that recognize six distinct sequences of the target.The optimized conditions for LAMP were studied using different Mg2+,dNTPs... |
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| Keywords: | Genetically modified maize Loop-mediated isothermal amplification(LAMP) Detection method |
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