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| 基因枪转化光温敏雄性不育小麦受体的选择与培养条件优化 | |
| 引用本文： | 王灵云,张立平,赵昌平,张风廷,樊建青,苑少华,郭玉平.基因枪转化光温敏雄性不育小麦受体的选择与培养条件优化[J].华北农学报,2011,26(4):126-129. |
| 作者姓名： | 王灵云 张立平 赵昌平 张风廷 樊建青 苑少华 郭玉平 |
| 作者单位： | 1. 首都师范大学生命科学学院,北京,100048 2. 首都师范大学生命科学学院,北京100048;北京杂交小麦工程技术研究中心,北京100097 3. 北京杂交小麦工程技术研究中心,北京,100097 |
| 基金项目： | 北京市自然科学基金项目(5091001); 国家高技术研究与发展计划“863”项目(2009AA101102;2011AA10A106); 农业部“948”项目(2009-Z4); 北京市农业育种基础研究创新平台项目(D08070500690801;D111100001311002) |
| 摘 要： | 为了选择适宜的基因枪转化光温敏雄性不育小麦的受体,并对幼胚组织培养条件进行优化,以小麦光温敏雄性不育系BS210、BS366以及常规品种京411的幼胚为材料,分别对碳源、除草剂浓度和基因型进行筛选.结果表明,在以蔗糖和麦芽糖为碳源的诱导培养基上,幼胚的出愈率差异不显著；在以蔗糖和麦芽糖为碳源的分化培养基上,愈伤组织的分... |
| 关 键 词： | 小麦幼胚 光温敏雄性不育 碳源 除草剂浓度 基因型 |
The Choice of Photo-thermo-sensitive Male Sterile Wheat Receptor Through Partical Bombardment and the Optimization of Culture Conditions |
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| WANG Ling-yun,ZHANG Li-ping,ZHAO Chang-ping,ZHANG Feng-ting,FAN Jian-qing,YUAN Shao-hua,GUO Yu-ping.The Choice of Photo-thermo-sensitive Male Sterile Wheat Receptor Through Partical Bombardment and the Optimization of Culture Conditions[J].Acta Agriculturae Boreali-Sinica,2011,26(4):126-129. | |
| Authors: | WANG Ling-yun ZHANG Li-ping ZHAO Chang-ping ZHANG Feng-ting FAN Jian-qing YUAN Shao-hua GUO Yu-ping |
| Institution: | WANG Ling-yun1,ZHANG Li-ping1,2,ZHAO Chang-ping1,ZHANG Feng-ting1,FAN Jian-qing1,YUAN Shao-hua2,GUO Yu-ping2(1.College of Life Science,Capital Normal University,Beijing 100048,China,2.Beijing Research Center for Hybrid Wheat,Beijing 100097,China) |
| Abstract: | In order to choose the appropriate receptor of photo-thermo-sensitive male sterile wheat for bombardment,and optimize the Culture condition of immature embryos,We used immature embryos of 3 wheat lines,including photo-thermo-sensitive male sterile line BS210,BS366 and normal cultivar Jing 411,Carbon source,screening concentration and genotype were screened respectively.The study showed that the frequency of embryos callus formation is not significantly different between sucrose and maltose as the carbon sou... |
| Keywords: | Wheat immature embryos Photo-thermo-sensitive male sterility Carbon source Concentration of herbicide Genotype |
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