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用于PCR快速检测的真菌基因组DNA制备研究进展
引用本文:崔菲,王子岚,高品红,王浩,杜克久.用于PCR快速检测的真菌基因组DNA制备研究进展[J].河北林果研究,2011,26(3):286-288.
作者姓名:崔菲  王子岚  高品红  王浩  杜克久
作者单位:河北农业大学林学院,河北 保定,071000
基金项目:国家自然科学基金项目(No.30972384)
摘    要:自然界中真菌种类很多,一些对人类有益,然而有很多会对人及动植物致病。对真菌的检测鉴定在医学、植物检疫以及真菌分类学研究方面具有重要的意义。PCR检测是目前真菌鉴定的常规检测手段,用于PCR检测的真菌基因组DNA制备是相关领域研究热点,对用于PCR快速检测的真菌基因组DNA制备方法进行综述。

关 键 词:真菌基因组DNA  快速制备  PCR检测

Research progress on the preparation of fungal genomic DNA for rapid PCR detection
CUI Fei,WANG Zi-lan,GAO Pin-hong,WANG Hao,DU Ke-jiu.Research progress on the preparation of fungal genomic DNA for rapid PCR detection[J].Hebei Journal of Forestry and Orchard Research,2011,26(3):286-288.
Authors:CUI Fei  WANG Zi-lan  GAO Pin-hong  WANG Hao  DU Ke-jiu
Institution:CUI Fei,WANG Zi-lan,GAO Pin-hong,WANG Hao,DU Ke-jiu(College of Forestry,Agricultural University of Hebei,Baoding 071000,China)
Abstract:There are many types of fungi in nature,some are benefit for people.But many are harmful to humans.Detection and identification of fungi in medicine,plant quarantine,and taxonomy of fungi have an important significance.PCR detection is the conventional means of identification Fungi,the preparation of fungal genomic DNA for rapid PCR detection is hot-spots in relevant flields.The method that the prearation of fungal genomic DNA for rapid PCR detection was reviewed in this paper.
Keywords:fungal genomic DNA  rapid preparation  PCR detection  
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