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骨形态发生蛋白基因4(BMP4)mRNA在蒙古牛不同组织的表达
引用本文:田蕾,王峰,班旭空,那顺温都乐,齐贵春,何小龙,田春英,刘永斌,荣威恒. 骨形态发生蛋白基因4(BMP4)mRNA在蒙古牛不同组织的表达[J]. 华北农学报, 2011, 26(4): 77-81
作者姓名:田蕾  王峰  班旭空  那顺温都乐  齐贵春  何小龙  田春英  刘永斌  荣威恒
作者单位:1. 锡林郭勒职业学院,内蒙古锡林浩特,026000
2. 内蒙古农牧业科学院,内蒙古呼和浩特,010031
3. 内蒙古财经学院,内蒙古呼和浩特,010010
4. 呼伦贝尔市鄂温克自治旗巴彦塔拉民族乡农牧业技术综合服务站,内蒙古呼伦贝尔,021107
5. 内蒙古农牧业科学院,内蒙古呼和浩特010031;内蒙古赛科星繁育生物技术股份有限公司,内蒙古呼和浩特010021
基金项目:内蒙古自然科学基金(2009BS0404); 国家科技支撑计划:草原肉牛肉羊绿色养殖关键技术集成研究与产业化示范
摘    要:蒙古牛是在蒙古高原的大陆性严寒气候条件下形成的一个古老的地方品种,它具有遗传性稳定、抗病耐粗饲、宜牧、抓膘能力强,适应性强等生物学特性.为了阐明BMP4基因对蒙古牛繁殖性能的影响,试验根据GenBank中报道的人、小鼠、绵羊和牛BMP4基因序列设计引物,从蒙古牛卵巢、输卵管、子宫、肾脏中提取RNA,以β-actin为内...

关 键 词:蒙古牛  BMP4  组织表达  半定量RT-PCR

The Expression of Bone Morphogenetic Protein-4 (BMP4) Gene in the Different Tissue of Mongolian Cattle
TIAN Lei,WANG Feng,BAN Xu-kong,NASHUNWENDOULE,QI Gui-chun,HE Xiao-long,TIAN Chun-ying,LIU Yong-bin,RONG Wei-heng. The Expression of Bone Morphogenetic Protein-4 (BMP4) Gene in the Different Tissue of Mongolian Cattle[J]. Acta Agriculturae Boreali-Sinica, 2011, 26(4): 77-81
Authors:TIAN Lei  WANG Feng  BAN Xu-kong  NASHUNWENDOULE  QI Gui-chun  HE Xiao-long  TIAN Chun-ying  LIU Yong-bin  RONG Wei-heng
Affiliation:TIAN Lei2,WANG Feng1,BAN Xu-kong4,NASHUNWENDOULE5,QI Gui-chun5,HE Xiao-long1,TIAN Chun-ying1,LIU Yong-bin1.3,RONG Wei-heng1(1.Inner Mongolia Academy of Agricultural and Animal Sciences,Huhhot 010031,China,2.Xilingol Vocational College,Xilinhot 026000,3.Inner Mongolia Skxing Reproductive Biotechnology Co.,Ltd,Huhhot 010021,4.Foreign Language Department of Inner Mongolia Finance and Economics College,Huhhot 010010,5.Service Station of Hulunbeier Ewenki Qi Agriculture and Animals Technolo...
Abstract:Mongolian cattle are ancient and local breed which were breed under cold weather condition of Mongolian Plateau.It possesses numerous outstanding characteristics such as,stable heredity,strong ability of disease resistance,coarse feed tolerance,grazing and fatten suitability,and great adaptability.In order to illustrate the influence of BMP4 gene to reproductive performance of Mongolian cattle,total RNA was isolated from the ovary,uterus,oviduct and kidney of Mongolian cattle,and the primers were designed a...
Keywords:Mongolian cattle  BMP4  Tissue expression  Semi-quantitative RT-PCR  
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