| 大豆灰斑病1号生理小种抗性基因的SSR标记分析 |
| |
| 引用本文： | 张文慧,陈庆山,杨庆凯,李文滨,王文辉,刘春燕,陈立君,刘海燕,单继勋.大豆灰斑病1号生理小种抗性基因的SSR标记分析[J].大豆科学,2004,23(3):169-173. |
| |
| 作者姓名： | 张文慧 陈庆山 杨庆凯 李文滨 王文辉 刘春燕 陈立君 刘海燕 单继勋 |
| |
| 作者单位： | 东北农业大学,哈尔滨市,150030 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 针对中国大豆灰斑病1号生理小种,以抗所有生理小种的品系东农40566为母本,以感1号生理小种的品种东农410为父本配制杂交组合,杂交得到F2代后连续自交3代得到F5代群体.该群体经人工接种灰斑病1号生理小种后,利用BSA法对500个SSR标记进行筛选,其中3个标记Satt565、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性,并且在F2代个体中表现出抗性与多态性协同分离的趋势.这3个标记与抗性基因的连锁顺序为Satt565-SOYGPATR-Hrcs1-Satt396,它们与抗性基因的连锁距离分别为12.7cM、6.5cM、14.7cM.推测抗大豆灰斑病1号生理小种的基因可能位于C1连锁群上.
|
| 关 键 词： | 大豆(G.max) 灰斑病(Cercospora sojina Hara) 抗性基因 SSR标记 |
| 文章编号： | 1000-9841(2004)03-0169-05 |
ANALYSIS OF RESISTANT GENE AGAINST CERCOSPORA SOJINA RACE 1 IN SOYBEAN WITH SSR MARKERS |
| |
| Zhang Wenhui,Chen Qingshan,Yang Qingkai,Li Wenbin,Wang Wenhui Liu Chunyan,Chen Lijun,Liu Haiyan,Shan Jixun.ANALYSIS OF RESISTANT GENE AGAINST CERCOSPORA SOJINA RACE 1 IN SOYBEAN WITH SSR MARKERS[J].Soybean Science,2004,23(3):169-173. |
| |
| Authors: | Zhang Wenhui Chen Qingshan Yang Qingkai Li Wenbin Wang Wenhui Liu Chunyan Chen Lijun Liu Haiyan Shan Jixun |
| |
| Abstract: | In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, bulked segregant analysis was applied to F 5 populations derived from cross between the resistant line Dongnong40566 and the susceptible line Dongnong410, self-crossing for three generations after F 2.Three SSR markers including Satt565,SOYGPATR and Satt396 were identified from 500 SSR markers, the linkage order is Satt565-SOYGPATR-Hrcs1-Satt396, and the distance to the resistance locus is 12.7, 6.5 ,14.7cM respectively. These three markers and the resistance locus were maped to soybean linkage group C1. |
| |
| Keywords: | Soybean (G max) Frogeye Leaf Spot(Cercospora sojina Hara) Resistant gene SSR marker |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
