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增铵营养对番茄幼苗生长和有机酸含量的影响
作者姓名:DONG Cai-Xi  SHEN Qi-Rong  WANG Ge
作者单位:[2]CollegeofResourcesandEnvironmentalSciences,NanjingAgriculturalUniversity,Nanjing210095(China)/E-mail:cxdong@njau.edu.cn
基金项目:*1Project supported by the National Natural Science Foundation of China (No.30270790) and National Post-doctoral Foundation of China (No.2003033494).
摘    要:A hydroponic experiment was conducted to study the effect of partial replacement of NO(3^-)-N by NH -N on theseedling growth and organic acid content of tomato (Lycopersicon esculentum Mill.). A completely randomized designwas established with three replications and five treatments, i.e., NO(3^-)-N/NH(4^ )-N of 100/0, 75/25, 50/50, 25/75 and0/100. Results showed that 25% replacement of NO(3^-)-N by NH(4^ )-N significantly (P = 0.05) improved fresh and dryweight, revealing that a proper percentage of NH -N was important for tomato nitrogen nutrition. This could increasethe plant growth even though tomato was a crop that preferred nitrate nutrition. Also an increase in the proportion ofNH -N in the nutrient solution led to a significant decrease (P=0.05) in malate, citrate and fumarate. However, the25% NH(4^ )-N plus 75% NO(3^-)-N treatment had no significant effect (P = 0.05) on the 2-ketoglutarate, succinate or oxalicacid content, showing that only some organic acids in tomato plants were affected. Only pyruvate increased significantly(P=0.05), and it only increased for 25% and 50% replacement of NO(3^-)-N by NH(4^ )-N. Metabolism of these organicacids, especially malate, citrate and fumarate, should be further studied at the molecular level in vegetables applied withdifferent nitrogen forms.

关 键 词:西红柿  生长  有机酸  离子化合物  溶液培养实验

Tomato growth and organic acid changes in response to partial replacement of NO3--N by NH4+-N
DONG Cai-Xi,SHEN Qi-Rong,WANG Ge.Tomato growth and organic acid changes in response to partial replacement of NO3--N by NH4+-N[J].Pedosphere,2004,14(2):159-164.
Authors:DONG Cai-Xi  SHEN Qi-Rong and WANG Ge
Institution:College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China). E-mail: cxdong@njau.deu.cn;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China). E-mail: cxdong@njau.deu.cn;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China). E-mail: cxdong@njau.deu.cn
Abstract:A hydroponic experiment was conducted to study the effect of partial replacement of NO(3^-)-N by NH -N on theseedling growth and organic acid content of tomato (Lycopersicon esculentum Mill.). A completely randomized designwas established with three replications and five treatments, i.e., NO(3^-)-N/NH(4^ )-N of 100/0, 75/25, 50/50, 25/75 and0/100. Results showed that 25% replacement of NO(3^-)-N by NH(4^ )-N significantly (P = 0.05) improved fresh and dryweight, revealing that a proper percentage of NH -N was important for tomato nitrogen nutrition. This could increasethe plant growth even though tomato was a crop that preferred nitrate nutrition. Also an increase in the proportion ofNH -N in the nutrient solution led to a significant decrease (P=0.05) in malate, citrate and fumarate. However, the25% NH(4^ )-N plus 75% NO(3^-)-N treatment had no significant effect (P = 0.05) on the 2-ketoglutarate, succinate or oxalicacid content, showing that only some organic acids in tomato plants were affected. Only pyruvate increased significantly(P=0.05), and it only increased for 25% and 50% replacement of NO(3^-)-N by NH(4^ )-N. Metabolism of these organicacids, especially malate, citrate and fumarate, should be further studied at the molecular level in vegetables applied withdifferent nitrogen forms.
Keywords:NH4+-N  nitrogen forms  NO3--N  organic acid  tomiato (Lycopersicon esculentum Mill  )
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