秋水仙素诱导林木多倍体研究进展

多倍体育种是创制和选育植物新品种的重要途径，秋水仙素是目前植物多倍体诱导使用最广泛和有效的试剂。文章综述了近年来秋水仙素在林木多倍体诱导方面的研究进展，归纳了林木多倍体诱导中诸如秋水仙素浓度、诱导时间、处理方法等影响因素及鉴定多倍体的方法。指出林木多倍体存在嵌合体分离纯化繁琐缓慢、新诱导剂亟需开发、林木多倍体评价周期长、同源多倍体分子机理研究不足等问题，进而对上述问题给出相应的解决策略和建议。该综述旨在为林木多倍体育种提供理论参考。

Polyploidy Induction by Colchicine in Forest Trees: Research Progress

Polyploid breeding is an important way to create and select new varieties of plants. Colchicine is the most widely used and effective reagent for plant polyploidy induction at present. In this paper, the research progress of colchicine in forest polyploidy induction in recent years is reviewed, and the influencing factors such as colchicine concentration, induction time, treatment methods and the methods for identifying polyploidy are summarized. It is pointed out that there are some problems in forest polyploidy, such as tedious and slow separation and purification of chimeras, urgent development requirement of new inducers, long evaluation period of forest polyploidy, and insufficient research on molecular mechanism of autopolyploid. Then, corresponding solutions and suggestions are given. The purpose of this review is to provide theoretical reference for forest polyploidy breeding.