| 载铜蒙脱石对病原菌黏附Caco-2细胞的影响 |
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| 引用本文： | 曹授俊,郭彤,李桂伶,关文怡. 载铜蒙脱石对病原菌黏附Caco-2细胞的影响[J]. 黑龙江畜牧兽医, 2011, 0(17) |
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| 作者姓名： | 曹授俊 郭彤 李桂伶 关文怡 |
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| 作者单位： | 北京农业职业学院; |
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| 基金项目： | 国家自然科学基金项目(30471255) |
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| 摘 要： | 为了探讨载铜蒙脱石(MMT-Cu)对大肠杆菌K88和猪霍乱沙门菌的黏附作用,试验采用Ca-co-2细胞培养模型,观察被标记的大肠杆菌K88、猪霍乱沙门菌对载铜蒙脱石的黏附作用;并在培养液中加入载铜蒙脱石,计算其对细菌黏附的阻断率,测定胞内乳酸脱氢酶(LDH)的释放情况。结果表明:测试菌与Caco-2细胞的黏附率分别为13.23%和10.78%;载铜蒙脱石对病原菌黏附Caco-2细胞均有不同程度的阻断作用,对大肠杆菌K88、猪霍乱沙门菌的阻断率分别为67.38%和60.45%,并能极显著降低LDH的释放(P<0.01)。说明载铜蒙脱石可有效阻断病原菌黏附,从而防治肠道细菌感染和细菌移位,可作为一种消化道黏膜保护剂。
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| 关 键 词： | 载铜蒙脱石 Caco-2细胞 黏附 大肠杆菌K88 猪霍乱沙门菌 |
Effects of copper-bearing montmorillonite on the adhesion of pathogenic bacteria to Caco-2 cells |
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| CAO Shou-jun,GUO Tong,LI Gui-ling,GUAN Wen-yi. Effects of copper-bearing montmorillonite on the adhesion of pathogenic bacteria to Caco-2 cells[J]. Heilongjiang Animal Science And veterinary Medicine, 2011, 0(17) |
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| Authors: | CAO Shou-jun GUO Tong LI Gui-ling GUAN Wen-yi |
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| Affiliation: | CAO Shou-jun,GUO Tong,LI Gui-ling,GUAN Wen-yi(Beijing Vocational College of Agriculture,Beijing 102442,China) |
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| Abstract: | Montmorillonite as a raw material,copper-exchanged montmorillonite(MMT-Cu) was prepared by using the method of cation exchange reaction.In order to investigate the adhesion of pathogenic strains to intestina epithelial cells and the effects of MMT-Cu on the bacterial adhesion,Caco-2 cell line was used as an in vitro model for intestinal epithelium and bacterial adhesion to Caco-2 cell cultures was quantitated using radiolabelled bacteria.The results showed that the adhesion percentage of Escherichia coli K8... |
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| Keywords: | copper-bearing montmorillonite Caco-2 cell adhesion Escherichia coli K88 S.choleraesuis |
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