| 金针菇固体培养几种胞外酶活力变化的研究 |
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| 引用本文： | 李蕤,吴克,骆军,张必武,潘仁瑞.金针菇固体培养几种胞外酶活力变化的研究[J].中国食用菌,2002,21(1):12-14. |
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| 作者姓名： | 李蕤 吴克 骆军 张必武 潘仁瑞 |
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| 作者单位： | 1. 中国科学技术大学生命科学学院;合肥联合大学化学生物工程系,合肥,230022
2. 合肥联合大学化学生物工程系,合肥,230022 |
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| 基金项目： | 安徽省教育厅自然科学基金资助项目 (2 0 0 0j12 6 7zc) |
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| 摘 要： | 本文主要研究了金针菇菌丝体在固体培养基上生长时胞外酶的活性变化。测定了木聚糖酶、羧甲基纤维素酶和淀粉酶的活性变化及蛋白质、还原糖的含量变化。并探讨了金针菇在改变了碳源的培养基上胞外酶、蛋白质、还原糖的变化。结果表明 :金针菇在固体培养过程中 ,三种酶均有活力 ,但呈现较大差异。培养过程中蛋白质和还原糖含量随时间变化 ,且与酶活力有关。改变固体培养基中的碳源 ,则胞外酶活性、蛋白质和还原糖含量又呈不同的变化趋势
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| 关 键 词： | 金针菇 木聚糖酶 羧甲基纤维素酶 淀粉酶 固体培养基 |
| 修稿时间： | 2001年5月14日 |
Study on Active Change of Various Exocellular Enzyme from Flammulina velutipes in Solid Culture Medium |
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| LI Rui,WU Ke,LUO Jun,ZHANG Bi-wu,PAN Ren-rui.Study on Active Change of Various Exocellular Enzyme from Flammulina velutipes in Solid Culture Medium[J].Edible Fungi of China,2002,21(1):12-14. |
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| Authors: | LI Rui WU Ke LUO Jun ZHANG Bi-wu PAN Ren-rui |
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| Abstract: | The change of various exocellular enzyme activity from Flammulina velutipes in the solid culture medium are studied.The content of xylanase carboxymethyl cellulose enzyme,diastase,protein and reducing sugar are detrmined and their activity changes are discussed.The result shows that all kinds of enzyme activity and the content of protein and reducing sugar are great difference.In addition,different changing tendency of the exocellular enzyme activity and the content of protein and reducing sugar after using another carbon source also are discussed. |
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| Keywords: | Flammulina velutipes Xylanase Carboxymethyl cellulose enzyme Diastase Solid culture medium |
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