| 紫花苜蓿光敏色素B基因片段克隆及RNA干扰表达载体的构建 |
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| 引用本文： | 朱见明,严学兵,史莹华,王成章.紫花苜蓿光敏色素B基因片段克隆及RNA干扰表达载体的构建[J].作物学报,2011,37(2):374-379. |
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| 作者姓名： | 朱见明 严学兵 史莹华 王成章 |
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| 作者单位： | 河南农业大学牧医工程学院, 河南郑州 450002 |
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| 基金项目： | 国家自然科学基金，教育部博士点基金 |
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| 摘 要： | 根据紫花苜蓿2级标准品种Vernal PhyB基因的序列(GenBank登录号为GQ379903.1)，设计2对含有酶切位点的特异性引物F1/R1和F2/R2，以紫花苜蓿总RNA为模板，通过RT-PCR法扩增得到PhyB基因正向、反向目的片段，将片段连接到pGEM-T Easy载体上得到重组载体pGEMB-1和pGEMB-2，再以中间载体pHANNIBAL和植物双元表达载体pART27为基础，通过多次酶切和连接，成功地构建了紫花苜蓿PhyB的RNAi表达载体。为进一步研究光敏色素B与苜蓿秋眠性之间的关系奠定了基础。
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| 关 键 词： | 紫花苜蓿 秋眠性 光受体 光敏色素 RNA干扰 |
| 收稿时间： | 2010-05-28 |
Cloning of Medicago sativa Phychrome B cDNA and Establishment of Its RNA Interference Expression Vector |
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| ZHU Jian-Ming,YAN Xue-Bing,SHI Ying-Hua,WANG Cheng-Zhang.Cloning of Medicago sativa Phychrome B cDNA and Establishment of Its RNA Interference Expression Vector[J].Acta Agronomica Sinica,2011,37(2):374-379. |
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| Authors: | ZHU Jian-Ming YAN Xue-Bing SHI Ying-Hua WANG Cheng-Zhang |
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| Institution: | Engineering College of Animal Husbandry and Veterinary Sciences, Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | The short day is one of the main factors affecting alfalfa fall dormancy,which is called as the photoperiodic effect.Study on the relationship between the main photoreceptors-PhyB gene and alfalfa fall dormancy may reveal the regulation mechanism of alfalfa fall dormancy radically,and provide a scientific reference for the application of alfalfa varieties differing in fall dormancy in forage production.The objective of this study was to establish an RNAi expression vector harboring PhyB gene of Medicago sat... |
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| Keywords: | Medicago sativa Fall dormancy Photoreceptor Phytochrome RNA interference |
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