外源哌啶酸对丽蝇蛹集金小蜂生长发育及耐寒性的影响

本研究以丽蝇蛹集金小蜂及其寄主红尾肉蝇为研究对象,评价了寄主饲料中添加哌啶酸对金小蜂生长发育和耐寒性的影响。结果表明,寄主饲料中添加哌啶酸的最适浓度为2%,无论金小蜂幼虫滞育与否,添加哌啶酸均能一定程度上降低金小蜂的鲜重、干重、含水量、水合量及过冷却点,其中对鲜重、含水量及水合量的影响显著。添加哌啶酸也能显著缩短非滞育金小蜂成蜂的寿命,降低其繁殖力,但对其发育历期和性比无显著影响。添加哌啶酸还能显著提高金小蜂幼虫的耐寒性,其中对滞育幼虫的耐寒性提高最显著。本研究结果证明哌啶酸对金小蜂的生长发育具有一定抑制作用,但可显著提高其耐寒性,这为深入研究哌啶酸的功能机制及促进金小蜂的利用提供了理论参考。

Effects of exogenous pipecolic acid on the development and cold tolerance of Nasonia vitripennis

In this study, we used the ectoparasitoid wasp Nasonia vitripennis and its host Sarcophaga crassipalpis to determine the effect of pipecolic acid added in host diet on the development and cold tolerance of N. vitripennis. The results showed that the optimum concentration of pipecolic acid added to the host diet was 2%. Pipecolic acid added in host diet could reduce the fresh mass, dry mass, water mass, hydration and supercooling points of the parasitoid larvae during diapausing or non-diapausing status. The fresh mass, hydration and supercooling point were significantly affected by exogenous pipecolic acid. Adding pipecolic acid in the host diet could significantly shorten the longevity of non-diapausing adults and reduce their fecundity, but no obvious effects on the developmental period and sex ratio were found. Cold tolerance of the parasitoid larvae were distinctly enhanced by adding pipecolic acid to the host diet, and the most significant effect was observed in the diapausing larvae. The results demonstrated that exogenous pipecolic acid had a negative effect on the development of N. vitripennis, but it could significantly elevate the cold tolerance of N. vitripennis, which provides a theoretical reference for further study of the functional mechanism of pipecolic acid and improves the use of N. vitripennis in the biological control program.