| 启动子Actin1的克隆及植物表达载体的构建 |
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| 引用本文： | 刘发央,侯路珍,谢小冬. 启动子Actin1的克隆及植物表达载体的构建[J]. 草原与草坪, 2005, 0(3): 66-68,71 |
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| 作者姓名： | 刘发央 侯路珍 谢小冬 |
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| 作者单位： | 甘肃农业大学,草业学院,甘肃,兰州,730070;兰州大学,生命科学学院,甘肃,兰州,730000 |
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| 基金项目： | 甘肃省亚盛集团资助项目 |
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| 摘 要： | 通过提取水稻叶片基因组DNA,设计PCR扩增特异引物,克隆单子叶特异表达启动子Ac-tin1基因,与T载体连接,转化E.coliDH5α,得到重组子,经酶切和序列分析鉴定,该片段分子大小为1238bp,通过序列对比分析发现与已发表的Actin1碱基序列有99.60%的同源性。特异启动子基因驱动的抗真菌肽(AFP)基因植物表达载体,就可以进行早熟禾黑麦草等单子叶牧草的遗传转化,为单子叶牧草和草坪草的抗真菌病研究提供方法。
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| 关 键 词： | 克隆 启动子 植物表达栽体 |
| 文章编号： | 1009-5500(2005)03-0066-03 |
| 修稿时间： | 2004-10-22 |
Cloning of monomial promoter of actin1 gene and construction of plant expression vector |
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| LIU Fa-yang,HOU Lu-zhen,XIE Xiao-dong. Cloning of monomial promoter of actin1 gene and construction of plant expression vector[J]. Grassland and Turf, 2005, 0(3): 66-68,71 |
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| Authors: | LIU Fa-yang HOU Lu-zhen XIE Xiao-dong |
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| Abstract: | Rice (Oryza sativa) genomic DNA was extracted from leaves. A 1238 bp monocotyledon express promoter Actin1 was cloned by PCR using artificial synthetic special primers and combined with plasmid pUCm-T easy vector and transformed E. coli DH 5α Sequence analysis indicated that the homology between this fragment and the reported sequence was 99.60%. This work was a foundation for exploring grass anti-fungal transformation. |
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| Keywords: | cloning promoter plant expression vector |
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