甘蓝型油菜显性核不育橙黄花纯合两型系的选育

以甘蓝型油菜显性核不育杂合两型系的不育株沪油15A为母本，与恢复系HF03杂交，F1群体全部表现为可育。在该杂交组合的6个F3群体中有5个群体发生育性分离。其中区号为04-4307的F3群体发生育性和花瓣色泽的分离，表现为70株鲜黄花可育、22株鲜黄花不育、30株橙黄花可育、9株橙黄花不育。根据油菜显性核不育和橙黄花的遗传规律，04-4307出现3:1育性分离，说明该群体由基因型为MsMsRfrf的单株自交获得。从04-4307群体中选橙黄花可育株自交，并与橙黄花不育株作兄妹交，后代全部表现为橙黄花(y1y1y2y2)。其中区号为05-4356的兄妹交组合出现1:1育性分离；其父本的可育株自交出现3:1育性分离。由此推断，05-4356的兄妹交组合就是显性核不育橙黄花纯合两型系，其不育株基因型为MsMsrfrfy1y1y2y2，可育株基因型为MsMsRfrfy1y1y2y2。

Developing a homozygous two-type line of dominant genic male sterility with orange flower in Brassica napus L.

A cross between heterozygous two-type line Huyou15A of dominant genic male sterility and restorer line HF03 led to all fertile F_1 plants. Five among 6 F_3 generation lines showed fertile segregation.A registered 04-4307 group showed a fertility segregation ratio at 3∶1,including 70 fertile with yellow flowers,30 fertile with orange flowers,22 sterile with yellow flowers and 9 sterile with orange flowers.The genotype of its F_2 plant should be MsMsRfrf. The fertile plants with orange flowers were self-pollinated and crossed with sister sterile plants with orange flowers. Both led to all orange flower offspring, while the sib-mating generation 05-4356 showed a fertility segregation ratio at 1∶1, and the self generation of corresponding fertile plant segregated at 3∶1.These results indicated the 05-4356 group to be a homozygous two-type line with orange flower developed by crossing sterile genotype MsMsrfrfy1y1y2y2 and fertile genotype MsMsRfrfy1y1y2y2.