首页 | 本学科首页   官方微博 | 高级检索  
     

转抗除草剂基因小麦植株的筛选方法研究
引用本文:周淼平,余桂红,任丽娟,朱伟芳,马鸿翔. 转抗除草剂基因小麦植株的筛选方法研究[J]. 麦类作物学报, 2008, 28(6)
作者姓名:周淼平  余桂红  任丽娟  朱伟芳  马鸿翔
作者单位:江苏省农业科学院农业生物技术研究所,江苏南京,210014;江苏省农业科学院农业生物技术研究所,江苏南京,210014;江苏省农业科学院农业生物技术研究所,江苏南京,210014;江苏省农业科学院农业生物技术研究所,江苏南京,210014;江苏省农业科学院农业生物技术研究所,江苏南京,210014
基金项目:江苏省自然科学基金 , 国家转基因生物新品种培育重大专项课题  
摘    要:为了降低小麦转bar基因时产生较高比例的假阳性植株,采用测定T0代转基因植株叶片释放NH4 浓度和对T1代植株喷施Basta除草剂等辅助筛选方法,建立了简便有效的转bar基因小麦筛选体系。研究结果表明,2叶期和5叶期叶片释放的NH4 浓度与bar基因PCR检测结果呈极显著相关,相关系数分别为0.86和0.75。对391株T0代再生植株进行叶片释放NH4 浓度测定,筛选到34株转基因植株,其中27株为bar基因的PCR检测所证实。用100 mg/L Basta除草剂对381株T0代植株的后代(3 800余株)进行喷施筛选,根据后代抗性分离筛选到13株T0代阳性植株,与PCR检测结果吻合度达到100%。这两种方法可用于以bar基因为选择标记基因的转基因植株的筛选。

关 键 词:小麦  bar基因  遗传转化  筛选

Screening of Transgenic Wheat Plants Resistant to Herbicide
ZHOU Miao-ping,YU Gui-hong,REN Li-juan,ZHU Wei-fang,MA Hong-xiang. Screening of Transgenic Wheat Plants Resistant to Herbicide[J]. Journal of Triticeae Crops, 2008, 28(6)
Authors:ZHOU Miao-ping  YU Gui-hong  REN Li-juan  ZHU Wei-fang  MA Hong-xiang
Abstract:Screening and identification of transgenic plants are the key factors for successful transformation.To decrease the "escape" plants in wheat transformation experiments,the detection of ammonium ion released from the leaves of T0 transgenic plants and spraying Basta herbicide on T1 transgenic plants were studied to establish a simple and efficient way to screen transgenic wheat plants with bar gene.The results showed the concentrations of ammonium ion from transgenic plants had significant correlations of r=0.86 at two-leaf stage,and r=0.75 at five-leaf stage with the result from PCR analysis.Thirty-four transgenic plants were obtained by ammonium assay from 391 T0 plants regenerated on selection medium,among which 27 transgenic plants were confirmed by PCR analysis for bar gene.Thirteen transgenic T0 lines obtained by spraying 100 mg/L Basta herbicide on the progenies derived from 381 T0 plants were all validated by PCR analysis.The result indicated that the two methods can be used to screen transgenic wheat plants when the bar gene is used as the selectable marker.
Keywords:Wheat  Bar gene  Transformation  Screening
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《麦类作物学报》浏览原始摘要信息
点击此处可从《麦类作物学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号