酵母激发子与水杨酸Ti转化的丹参组培物内源激素含量的影响

Ti转化的丹参组培物在MS－HN4培养基（MS中不含硝酸铵，含蔗糖30g.L^-1)上经25度暗培养18d，生长量由大到小依次为：对照（CK），200umol.L^-1水杨酸（SA），4g.L^-1酵母激发子（E）和E＋SA处理，其中CK和SA处理的组培物仅产生痕量的丹参酮类物质，而E和E＋SA处理的总丹参酮产量分别增加到12．23mg.L^-1和15．07mg.L^-1，表明E处理能促进组培物产生丹参酮类物质，而A可以强化E的这种作用，与上术差异有关的内源激素变化是：培养6－18d ,E SA处理的ABA和iPAs含量均持续高于对照，分别增长了2．8－9．8倍和3．6－5．8倍，而A1＋3和IAA 含量分别比对照降低13．2％－56．9％和34．8％－74．6％，高水平的ABA和iPAs及低水平GA1＋3和IAA抑制了组培物的生长，有利于促进丹参酮产量的提高。

Effect of yeast elicitor and salicylic acid on contents of endogenous plant hormones in Ti-transformed Salvia miltiorrhiza cell aggregates

The fresh weight and dry weight of Ti-transformed Salvia miltiorrhiza cell aggregates increase differently with various treatm ents,after being cultured in the MS-NH4 medium(MS without ammonium nitrate,co ntaining 30 g*L－1 sucrose)under darkness for 18 days(25 ℃).The total tanshinones production reaches 12.23 mg*L－1 and 15.07 mg*L－1 by exogenous application of 4 g*L－1 of yeast elicitors(E)and E+200 μmol*L－1 of salicylic acid(SA)respectiv ely.Howeve r,only trace amount of tanshinones was detected in the control and SA treatment, indicating that E could promote tanshinones production and SA enhances the effect of E.Compared with the control,the contents of ABA and iPAs in E+SA treated cell aggregates rise continuously and with an increase of 2.8-9.8 and 3.6-5. 8 fold respectively,but those of GA1+3 and IAA are decreased by 13.2%-56 .9% and 34.8%-74.6% respectively,suggesting that high level of ABA and iPAs combined with low level of GA1+3 and IAA inhibits the growth of cell aggre gates,and therefore significantly stimulates the tanshinones production.