| 斑玉蕈甘油醛-3-磷酸脱氢酶基因克隆与序列分析 |
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| 引用本文： | 张津京,陈辉,冯志勇,陈明杰,汪虹. 斑玉蕈甘油醛-3-磷酸脱氢酶基因克隆与序列分析[J]. 食用菌学报, 2011, 18(2): 5-9 |
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| 作者姓名： | 张津京 陈辉 冯志勇 陈明杰 汪虹 |
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| 作者单位： | 1. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403; 南京农业大学生命科学学院,南京210095
2. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403 |
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| 基金项目： | 国家科技支撑计划项目(编号:2010BAK69B18); 上海科委科研平台建设专项(编号:09dz2200800); 上海市科委崇明科技攻关专项(编号:10DZ1960100)的部分研究内容 |
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| 摘 要： | 甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)是糖酵解和卡尔文循环中的关键酶,本实验室之前的研究发现斑玉蕈菌丝的GAPDH表达随培养基中葡萄糖添加浓度的变化而变化,在此基础上我们首次克隆了斑玉蕈GAPDH的DNA和cDNA序列,结果斑玉蕈gpd基因长2 724 bp,对比基因组DNA和cDNA序列知其中有7个内含子、8个外显子;其理论分子量为36.15 kD、编码蛋白质含338个氨基酸、等电点(PI)为8.24。
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| 关 键 词： | 斑玉蕈 甘油醛-3-磷酸脱氢酶 基因克隆 序列分析 |
Cloning and Sequence Analysis of a Glyceraldehyde 3-Phosphate Dehydrogenase Gene from Hypsizygus marmoreus |
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| ZHANG Jinjing,CHEN Hui,FENG Zhiyong,CHEN Mingjie,WANG Hong. Cloning and Sequence Analysis of a Glyceraldehyde 3-Phosphate Dehydrogenase Gene from Hypsizygus marmoreus[J]. Acta Edulis Fungi, 2011, 18(2): 5-9 |
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| Authors: | ZHANG Jinjing CHEN Hui FENG Zhiyong CHEN Mingjie WANG Hong |
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| Affiliation: | ZHANG Jinjing1,2,CHEN Hui1,FENG Zhiyong1,CHEN Mingjie1,WANG Hong1 1National Research Center for Edible Fungi Biotechnology and Engineering,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China,2College of Life Sciences,Nanjing Agricultural University,Nanjing 210095 |
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| Abstract: | A glyceraldehyde-3-phosphate dehydrogenase gene(gpd) and corresponding cDNA were cloned from the edible fungus,Hypsizigus marmoreus.Comparison of the cloned DNA and cDNA sequence revealed that gpd was 2 724 bp in length,containing seven introns and eight exons,and encoding a protein consisting of 338 amino acids residues.The calculated molecular weight and PI of the protein were 36.15 kD and pH 8.24,respectively. |
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| Keywords: | Hypsizigus marmoreus Glyceraldehyde-3-phosphate dehydrogenase gene cloning sequence analysis |
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