|
|||||
|
|
| HPLC法测定香菇中香菇嘌呤含量 | |
| 引用本文： | 王淑蕾,梁敬钰,唐庆九,刘艳芳,周帅,杨焱,张劲松. HPLC法测定香菇中香菇嘌呤含量[J]. 食用菌学报, 2011, 18(2): 49-56 |
| 作者姓名： | 王淑蕾 梁敬钰 唐庆九 刘艳芳 周帅 杨焱 张劲松 |
| 作者单位： | 1. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海204303;中国药科大学中药学院,江苏南京210009 2. 中国药科大学中药学院,江苏南京,210009 3. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海204303 |
| 基金项目： | 上海市科技兴农重点攻关项目[编号:沪农科攻字(2009)第2-2号]的部分研究内容~~ |
| 摘 要： | 建立1种运用高效液相色谱(HPLC)测定香菇(Lentinula edodes)中香菇嘌呤含量的方法。优化后的色谱条件为:Ultimate AQ-C18柱(5μm,4.6 mm×250 mm),流动相为甲醇-磷酸盐缓冲液(7∶93,pH 4.67)等度洗脱,流速1 mL/min,柱温30℃,检测波长259 nm,进样量10μL。研究结果发现该方法准确、灵敏、重现性好,适用于香菇嘌呤的定量分析。 |
| 关 键 词： | 香菇 香菇嘌呤 HPLC |
Determination of Eritadenine in Lentinula edodes Fruit bodies Using HPLC |
|
| WANG Shulei,LIANG Jingyu,TANG Qingjiu,LIU Yanfang,ZHOU Shuai,YANG Yan,ZHANG Jingsong. Determination of Eritadenine in Lentinula edodes Fruit bodies Using HPLC[J]. Acta Edulis Fungi, 2011, 18(2): 49-56 | |
| Authors: | WANG Shulei LIANG Jingyu TANG Qingjiu LIU Yanfang ZHOU Shuai YANG Yan ZHANG Jingsong |
| Affiliation: | WANG Shulei1,2,LIANG Jingyu2,TANG Qingjiu1,LIU Yanfang1,ZHOU Shuai1,YANG Yan1,ZHANG Jingsong1 1National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China,2 Traditional Chinese Medicine College,China Pharmaceutical University,Nanjing,Jiangsu 210009 |
| Abstract: | A simple and sensitive HPLC-based method for the accurate determination of eritadenine in Lentinula edodes fruit bodies is described.Optimization of sample work-up procedures showed that the efficiency of eritadenine extraction was highest when powdered fruit bodies were suspended in 5%(w/v) ethanol and subjected to ultrasound for 30 min using a 1∶100 sample-solvent ratio.Optimal separation of eritadenine in the extracts was achieved by loading 10 μL samples on to an Ultimate AQ-C18 column(5 μm,4.6 mm×250 mm) and eluting with methanol-phosphate buffer(7∶93,pH 4.67) at 30 ℃ using a flow rate of 1.0 mL/min.Eritadenine in eluted fractions was monitored at 259 nm. |
| Keywords: | Lentinula edodes eritadenine HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |