| 降香黄檀、檀香根际解钾菌的筛选与活性研究 |
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| 引用本文： | 罗娜,周德明,徐睿,周国英.降香黄檀、檀香根际解钾菌的筛选与活性研究[J].热带作物学报,2016,37(5):964-970. |
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| 作者姓名： | 罗娜 周德明 徐睿 周国英 |
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| 作者单位： | 1. 中南林业科技大学/经济林培育与保护教育部重点实验室,湖南长沙,410004;2. 中南林业科技大学/森林有害生物防控湖南省重点实验室,湖南长沙,410004 |
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| 基金项目： | 国家林业公益性行业科研专项（No. 201304402）。 |
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| 摘 要： | 为提高降香黄檀-檀香人工林的土壤肥力,从不同林龄的降香黄檀、檀香根际土壤中分离筛选高效解钾菌。从钾细菌富集培养基上初筛和解钾菌筛选培养基复筛得到可培养物89个。采用原子吸收分光光度法测定菌株解钾能力。结果表明,可溶性钾含量在70μg/m L以上的菌株有6株,其中菌株JT-K21、JT-K11和JT-K18的解钾率为80%以上。JT-K21培养液的可溶性钾含量高达132.68μg/m L,解钾率达到221.18%。基于形态学、生理生化特性及16S rDNA序列比较分析,初步鉴定JT-K21为恶臭假单胞菌Pseudomonas putida。不同碳氮源试验表明:JT-K21对蔗糖和硫酸铵的吸收效果最好。通过单因素试验及正交试验结果表明:菌株JT-K21液体发酵菌体浓度最大培养组最优组分为蔗糖(10 g/L)、硫酸铵(0.2 g/L)、氯化钠(0.1 g/L)、钾长石(5 g/L);JT-K21液体发酵解钾活性最佳时,培养基最优组分为蔗糖(10 g/L)、硫酸铵(0.2 g/L)、氯化钠(0.1 g/L)、钾长石(3 g/L)。
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| 关 键 词： | 降香黄檀 檀香 解钾菌 |
Screening and Identification of Potassium Bacteria in the Rhizosphere of Dalbergia Odorifera, Santalum album Land the ActivityAssay |
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| LUO N,ZHOU Deming,XU Rui and ZHOU Guoying.Screening and Identification of Potassium Bacteria in the Rhizosphere of Dalbergia Odorifera, Santalum album Land the ActivityAssay[J].Chinese Journal of Tropical Crops,2016,37(5):964-970. |
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| Authors: | LUO N ZHOU Deming XU Rui and ZHOU Guoying |
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| Institution: | Key Laboratory of Education for Non-timber Product Forest Silviculture and Protection, Central South University of Forestry and Technology;Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology;Key Laboratory of Education for Non-timber Product Forest Silviculture and Protection, Central South University of Forestry and Technology;Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology |
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| Abstract: | |
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| Keywords: | Dalbergia Odorifera Santalum album L Potassium releasing bacteria |
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