绵羊精液不同处理方法对体外受精效果的影响

试验通过开展绵羊低温保存精液和冷冻精液制备精子对体外受精效果影响的研究,旨在探讨制备绵羊IVF优质精液的新方法。结果表明:利用低温保存精液和冷冻精液,分别采用Percoll离心法和上游法制备精子进行体外受精,低温保存精液上游法的卵裂率78.6%显著高于低温保存精液和冷冻精液Percoll离心法和60.2%、62.3%,(P<0.05),极显著高于冷冻精液上游法21.1%,P<0.01;低温保存精液和冷冻精液Percoll离心法组间无显著(P>0.05)差异,但均显著(P<0.05)高于冷冻精液上游法。低温保存绵羊精液可用于制备IVF精子,上游法优于Percoll离心法,可显著提高体外受精效果。

Effect of Sheep Semen Different Treatment Methods on in vitro Fertility

In order to provide some important data for IVF technique,the effect of cryopreservation and cold preservation sheep semen on the cleavage rate in vitro fertilization were studied.The results showed that,the cleavage rate of swimming-up cold preservation semen78.6% was higher significantly than that of cryopreservation or cold preservation semen by percoll-density-gradient-centrifugation method 60.2% and 62.3%(P<0.05),and cryopreservation semen by the same method 21.1%(P<0.01);the cleavage rates had no obvious different between cold preservation and cryopreservation semen preparation with the percoll-density-gradient-centrifugation method(P>0.05),but they were significantly higher than those of swimming-up cryopreservation semen(P<0.05).In conclusion,cold preservation sheep semen was a very good method of preparation IVF sperm,swimming-up cold preservation semen was better than percoll-density-gradient-centrifugation method.