从马铃薯上分离出一种脉暗病毒

 从大名红皮花皱叶植株上通过蚜虫分离出一个分离物,能侵染许多茄科植物,在直果蔓陀蘿上表现为明显的脉暗症状。
经测定致死温点为55-56℃,稀释限点为200-300倍,适宜的酸碱度为pH6-9,在22℃下体外存活期6小时,在15-25℃下经过25天的自然干燥仍保有活力。抗药力极强,能抗85%酒精、50%硫酸铜液、19%甲醛液、2.5%单宁酸,而不失其致病力。桃蚜、棉蚜、玉米蚜、麦二义蚜、麦长鬚蚜都能传播此病毒。蚜虫在病株上吸食10分钟即能带毒,带毒的蚜虫在寄主植物上放饲10分钟以上即可传染,其传毒能力与虫口密度有密切关系,每次需有10头以上才能传染。本病毒在洋酸浆、直果蔓陀蘿、龙葵等指示植物上的病状与PYV所引起的症状有显著区别,对PYV的抗血清无明显的沉淀反应,在洋酸浆上与PYV之间的交互保护作用很小。

STUDIES ON A VEIN-BANDING VIRUS ISOLATED FROM POTATO PLANTS

With the aid of aphids (Myzus persicae),a new virus has been isolated from the crinkled potato plants of Daming Early Red-Skinned variety.On sap inoculation,it infects plants be-longing to many genera and species of Solanaceae.Datura stramonium shows clear mosaid and vein-banding symptoms.
The thermal death point is 55-56℃ 10 min.The dilution-end point is 1:200-300.The range of optimal pH is 6-9.The stability in vitro is about 6 hrs.at 22℃.Virulence main-tains under dry conditions at room temperature (15-25℃) for 25 days.Infectivity is not lost when treated with 85% ether alcohol,50% CuSO₄,19% formaldehyde and 25% tannic acid.