Transmission by aphids of potato spindle tuber viroid encapsidated by potato leafroll luteovirus particles |
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Authors: | Jerzy Syller Waldemar Marczewski Jerzy Paw?owicz |
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Institution: | (1) Centre M ochów, Potato Research Institute, 05- 832 Rozalin, Poland;(2) Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland |
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Abstract: | Potato spindle tuber viroid (PSTVd) was transmitted by Myzus persicae to Physalis floridana from P. floridana plants that also were infected with potato leafroll luteovirus (PLRV), whereas it was not transmitted by aphids from plants infected with PSTVd alone. Dot-blot hybridisation was used to detect PSTVd. The results indicate that PLRV can assist PSTVd in its transmission by M. persicae. Doubly infected, aphid-inoculated P. floridana plants from the previous experiment were used as the source plants in aphid transmission tests to the tomato cv. Rutgers, P. floridana and Datura stramonium. PSTVd was detected in 17 of 30 plants of tomato. The viroid was not detected by dot-blotting in any plant of P. floridana and D. stramonium in this experiment, but it was recovered from some plants by sap inoculation of the Rutgers plants. Treatment with RNase A of PLRV preparations purified from doubly infected plants indicated that PSTVd was encapsidated by PLRV particles. |
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Keywords: | dot-blot hybridisation ELISA in vivo encapsidation mixed infection Myzus persicae |
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