Isolation and identification of anaerobic bacteria from ovine foot rot in Spain |
| |
Authors: | S Piriz Duran R Cuenca Valera J Valle Manzano S Vadillo Machota |
| |
Institution: | Unidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo, Cáceres, Spain. |
| |
Abstract: | A microbiological study was made of 125 Merino sheep showing clinical signs of foot rot. A total of 435 strictly anaerobic strains were isolated, belonging to the following genera: Bacteroides, Peptostreptococcus, Tissierella, Fusobacterium, Megasphaera, Eubacterium, Acidaminococcus, Clostridium, Peptococcus and Propionibacterium. Of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: Bacteroides nodosus, B putredinis, B buccae, B ruminicola subspecies brevis, Tissierella praeacuta, Peptostreptococcus anaerobius and Megasphaera elsdenii. Six culture media were used for isolation. Agar brucella and agar brucella enriched with G-N anaerobe supplement proved to be the most efficient for isolating anaerobic bacteria. |
| |
Keywords: | |
|
|