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| 玉米ZmERD4基因的分离及转基因株系的获得 | |
| 引用本文： | 曹言勇,赵心萍,赵秋勇,张艳,唐保军,刘方方,王利锋,李会勇. 玉米ZmERD4基因的分离及转基因株系的获得[J]. 河南农业科学, 2011, 40(12): 43-47 |
| 作者姓名： | 曹言勇 赵心萍 赵秋勇 张艳 唐保军 刘方方 王利锋 李会勇 |
| 作者单位： | 1. 河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,河南郑州,450002 2. 河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,河南郑州 450002;太原理工大学化工学院,山西太原 030024 3. 太原理工大学化工学院,山西太原,030024 |
| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08009-061B);河南省科技攻关项目(092102110039) |
| 摘 要： | 为了获得转ZmERD4基因玉米植株,验证ZmERD4在玉米育种中的应用价值,通过同源克隆的方法在抗旱性强的玉米自交系CN165中克隆了ZmERD4基因全长cDNA,该基因全长2 073 bp,包含一个编码591个氨基酸的开放阅读框；推导的氨基酸序列和水稻中的OsERD4(XP476646)有82％的相似性,与拟南芥中的... |
| 关 键 词： | 玉米 ZmERD4 植物转化载体 转基因株系 |
Isolation of ZmERD4 Gene and Generation of Transgenic Maize Plants |
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| CAO Yan-yong , ZHAO Xin-ping , ZHAO Qiu-yong , ZHANG Yan , TANG Bao-jun , LIU Fang-fang , WANG Li-feng , LI Hui-yong. Isolation of ZmERD4 Gene and Generation of Transgenic Maize Plants[J]. Journal of Henan Agricultural Sciences, 2011, 40(12): 43-47 | |
| Authors: | CAO Yan-yong ZHAO Xin-ping ZHAO Qiu-yong ZHANG Yan TANG Bao-jun LIU Fang-fang WANG Li-feng LI Hui-yong |
| Affiliation: | 1(1.Cereal Crop Research Institute,Henan Provincial Key Laboratory for Maize Biology,Henan Academyof Agricultural Sciences,Zhengzhou 450002,China;2.College of Chemical Engineering,Taiyuan University of Technology,Taiyuan 030024,China) |
| Abstract: | Using homology cloning method,the full length cDNA of ZmERD4 was isolated from inbred line CN165 of drought resistant maize.The length of ZmERD4 was 2073bp,and the predicated protein comprised of 591 amino acids.Bioinformatics analysis indicated that the amino acids shared 82% and 58% similarity with rice OsERD4(XP476646)and Arabidopsis AtE4RD4(BAB63915),respectively.The plant transformation vector,in which ZmERD4 was driven by cauliflower mosaic virus 35S promoter,was conducted and transformed into the immature embryos of maize hybrid Hi-Ⅱ through Agrobaterium-mediated transformation system.Transgenic maize plants were assayed by bialaphos-resistance screening and PCR amplification of the bialaphos-resistance gene and ZmERD4 gene.The results showed that 42 transgenic maize lines with ZmERD4 were obtained,and the average transformation efficiency was 2%,which provided the important material basis for drought resistant maize breeding. |
| Keywords: | Maize(Zea mays) ZmERD4 Plant transformation vector Transgenic lines |
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