利用电极盘进行猪卵母细胞电激活 |
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引用本文: | 张玲,黄友防,何若钢,卢晟盛,许惠艳,兰宗宝,房慧伶,潘天彪,蓝海恩,黄敏瑞,卢克焕. 利用电极盘进行猪卵母细胞电激活[J]. 安徽农业科学, 2009, 37(15): 6999-7000 |
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作者姓名: | 张玲 黄友防 何若钢 卢晟盛 许惠艳 兰宗宝 房慧伶 潘天彪 蓝海恩 黄敏瑞 卢克焕 |
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作者单位: | 1. 广西大学广西亚热带生物资源保护利用重点实验室,广西,南宁,530005;广西大学动物科学技术学院,广西,南宁,530005 2. 广西大学动物科学技术学院,广西,南宁,530005 3. 广西畜牧研究所,广西,南宁,530001 |
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基金项目: | 国家自然科学基金,广西科学研究与技术开发计划项目,广西亚热带生物资源保护利用重点实验室开放课题 |
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摘 要: | [目的]研究不同电激活参数以及电脉冲联合6-DMAP对猪卵母细胞孤雌激活效果的影响。[方法]使用电极盘,测定不同场强(1.1、1.3、1.5、1.7、1.9kV/cm),不同脉冲时程(30、50、70、90、110ps),1次脉冲下猪卵母细胞囊胚率的变化。[结果]结果表明,脉冲强度以1.5和1.7kV/cm效果最好,在80胂时其猪卵母细胞囊胚率分别达到(22.35±5.16)%和(20.59±9.41)%;脉冲强度1.5kWcm,1次电脉冲后4h联合6-DMAP激活卵母细胞,当脉冲时程达到70和90μs时,囊胚率分别达到(21.65±9.95)%和(23.10±16.27)%。[结论]在试验条件下,使用电极盘的最适电激活参数为脉冲强度1.5-1.7kV/cm,脉冲时长80μs,1次脉冲电激活。
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关 键 词: | 猪 卵母细胞 孤雌激活 |
Electrical Activation of Porcine Oocytes with Electropolar Plates |
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Affiliation: | ZHANG Ling et al ( Guangxi Key Labomtory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning, Guangxi 530005) |
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Abstract: | [Objective]The research aimed to study the effects of different field strength and electricity combined with 6-dimethylaminopurine(6-DMAP) on the parthenogenetic development of porcine oocytes using electropolar plate.[Method]Porcine oocytes blastocyst rates were measured under the situations of different field strength(1.1,1.3,1.5,1.7,1.9 kV/cm),and different pulse duration(30,50,70,90,110 μs),one times of pulse with electropolar plate.[Result]The results showed that,1.5 and 1.7 kV/cm field strength were better,and the porcine oocytes blastocyst rates was(22.35±5.16)% and(20.59±9.41)% with duration of 80 μs.Treated with 1.7 kV/cm field strength,one times of pulse and combined with 6-DMAP 4 h later,the porcine oocytes blastocyst rates was(21.65±9.95)% and(23.10±16.27)% when pulse duration was 70 and 90 μs.[Conclusion]In conclusion,the best electrical parameters for porcine oocyte parthenogenesis was 1.5-1.7 kV/cm with one 80 μs pulse when using electropolar plate. |
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Keywords: | Porcine Oocyte Parthenogenetic activation |
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