| 水分胁迫下玉米叶片中一氧化氮与钙和钙调素的相互作用 |
| |
| 引用本文： | 桑建荣,蒋明义,陈永昌.水分胁迫下玉米叶片中一氧化氮与钙和钙调素的相互作用[J].南京农业大学学报,2010,33(5). |
| |
| 作者姓名： | 桑建荣 蒋明义 陈永昌 |
| |
| 作者单位： | 1. 江苏大学基础医学与医学技术学院,江苏,镇江,212013
2. 南京农业大学生命科学学院,江苏,南京,210095 |
| |
| 基金项目： | 国家自然科学基金项目(30571122) |
| |
| 摘 要： | 以玉米(Zea mays L.)为材料,研究水分胁迫下玉米叶片中NO与Ca2+和钙调素(CaM)含量之间的关系。结果显示:Ca2+鳌合剂、Ca2+通道阻塞剂和CaM拮抗剂预处理均抑制了水分胁迫诱导的NO产生和一氧化氮合酶(nitric ox-idesynthase,NOS)活性的提高,同时也能抑制水分胁迫诱导的叶片线粒体NO产生,而对叶绿体NO产生并没有影响;NO清除剂羟基2苯基4,4,5,5四咪唑1羟基3氧化物(2-4-carboxypheny l-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide,c-PTIO)能抑制水分胁迫条件下Ca2+和CaM的产生以及CaM1基因表达,但对水分胁迫最初诱导的玉米叶片叶肉细胞原生质体Ca2+和玉米叶片中CaM1基因表达以及CaM含量增加没有影响。上述结果表明:水分胁迫下NO和Ca2+/CaM之间存在相互作用。
|
| 关 键 词： | Ca2 /CaM NO NOS 水分胁迫 玉米 |
Interplay between nitric oxide and calcium/calmodulin in maize leaves (Zea mays L.) exposed to water stress |
| |
| SANG Jian-rong,JIANG Ming-yi,CHEN Yong-chang.Interplay between nitric oxide and calcium/calmodulin in maize leaves (Zea mays L.) exposed to water stress[J].Journal of Nanjing Agricultural University,2010,33(5). |
| |
| Authors: | SANG Jian-rong JIANG Ming-yi CHEN Yong-chang |
| |
| Institution: | SANG Jian-rong1,JIANG Ming-yi2,CHEN Yong-chang1 ( 1. School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang 212013,China,2. College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China) |
| |
| Abstract: | In this study,the relationship between calcium ( Ca2 + ) /calmodulin ( CaM) levels and NO production was investigated in leaves of maize ( Zea mays L. ) exposed to water stress. Pretreatments with Ca2 + inhibitors or CaM antagonists inhibited water stress-induced NO production and the activity of NOS in cytosolic and microsomal fractions of maize leaves. Interestingly, blockade of Ca2 + /CaM with Ca2 + inhibitors or CaM antagonists could only reduce NO level in mitochondria but not in chloroplast. Exogenous... |
| |
| Keywords: | calcium-calmodulin nitric oxide nitric oxide synthase water stress Zea mays L |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
