| K88-ST融合基因的克隆及真核表达载体的构建 |
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| 引用本文： | 任雪艳,包慧芳,陈创夫,孔庆军.K88-ST融合基因的克隆及真核表达载体的构建[J].猪业科学,2004,21(11):1-4. |
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| 作者姓名： | 任雪艳 包慧芳 陈创夫 孔庆军 |
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| 作者单位： | 石河子大学生物工程学院新疆地方与民族高发病重点实验室,新疆,石河子,832003;新疆石河子大学动物科技学院,新疆,石河子,832003 |
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| 摘 要： | 利用DNA重组技术,将编码K88-ST融合基因的序列片段克隆到植物表达载体pCAMBIA1302中,成功的构建了植物重组表达质粒pCAMBIA-K88-ST。并将其转化农杆菌EHA105,为K88-ST基因的进一步研究奠定基础。
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| 关 键 词： | K88-ST融合基因 植物重组表达质粒 基因克隆 |
| 文章编号： | 1008-9381(2004)11-0001-04 |
| 修稿时间： | 2004年9月10日 |
Constructiojn of the Recombinant Plasmid pCAMBIA-K-ST |
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| Ren Xue-yan,Bao Hui-fang,Chen Chuang-fu,Kong Qing-jun.Constructiojn of the Recombinant Plasmid pCAMBIA-K-ST[J].Swine Industry Science,2004,21(11):1-4. |
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| Authors: | Ren Xue-yan Bao Hui-fang Chen Chuang-fu Kong Qing-jun |
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| Institution: | Ren Xue-yan1 Bao Hui-fang1 Chen Chuang-fu2 Kong Qing-jun1 |
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| Abstract: | The plant recombinant expression plasmid pCAMBIA-K-ST has been successfully constructed by cloning the gene of K88-ST into the plant expression vector pCAMBIA1302. The molecular biological technique such as single-enzyme-insertion was also used in this research. The plant binary expression vector of K88-ST was transformed into A grobacterium EHA 105. It provided a reliable basis for the further study on K88-ST. |
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| Keywords: | K88-ST gene plant recombination expression plasmid gene cloning |
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