藏药短穗兔耳草中微量元素的形态分析 |
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引用本文: | 宋青云,肖昭蓉,程湘宏,刘占厚,陈晓红,钱蔚,严海英. 藏药短穗兔耳草中微量元素的形态分析[J]. 安徽农业科学, 2010, 38(27): 14937-14939. DOI: 10.3969/j.issn.0517-6611.2010.27.047 |
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作者姓名: | 宋青云 肖昭蓉 程湘宏 刘占厚 陈晓红 钱蔚 严海英 |
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作者单位: | 青海大学医学院药学系,青海西宁,810001;青海岩矿测试应用研究所,青海西宁,810008 |
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基金项目: | 青海大学医学院科学技术研究项目 |
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摘 要: | [目的]建立常用的藏药短穗兔耳草的水煎液和人工胃液中Ca、Fe、Mg、Mn、Zn、Cu、Pb和As8种微量元素的形态分析方法。[方法]利用HNO3-HClO4湿法消解样品,采用0.45μm微孔滤膜分离水煎液中的可溶态和悬浮态微量元素;利用D101大孔吸附树脂柱分离有机态和无机态微量元素;同时制备了样品的人工胃液提取液。采用ICP-OES法依次对原生药及各种形态中的8种元素含量进行测定。[结果]短穗兔耳草中8种元素在原生药、可溶态和人工胃液提取液中的形态分布基本一致。水溶液的提取率在1.48%~44.87%,人工胃液中的溶出率在2.45%~62.82%。方法的加标回收率在92.7%~104.5%,相对标准偏差小于2.48%。[结论]该研究建立的方法可同时快速、简便、准确地测定短穗兔耳草中各形态的8种微量元素,且短穗兔耳草中含有丰富的人体必需微量元素Ca、Mg、Fe、Zn和Mn,可能是其发挥药效的重要原因之一。
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关 键 词: | 藏药 短穗兔耳草 微量元素 形态分析 ICP-OES |
Speciation Analysis on Trace Elements in Lagotis brachystachya Maxim |
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Affiliation: | SONG Qing-yun et al(School of Pharmacy,Medical College,QingHai University,Xining,Qinghai 810001) |
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Abstract: | [Objective] To develop methods for speciation analysis of trace elements in aqueous solution and simulated gastric fluid of Lagotis brachystachya Maxim,a kind of Tibetan medicines.[Method] Samples were digested by HNO3-HClO4.The water-soluble state and suspension state were separated through microporous filtering film.Water-soluble state was divided into organic state and inorganic state with macroporous adsorption resin.Artificial gastric juice was prepared for extracting trace elements from the samples.The elements were detected by ICP-OES.[Result] The species distribution of the eight trace elements was consistent in the original sample,water-soluble state and gastric juice.The extraction rate of elements was 1.48%-44.87% in the aqueous solution and 2.45%-62.82% in the simulated gastric fluid.The recovery rate of standard addition was 92.7%-104.5%,and the relative standard deviations(RSD)were less than 2.48%.[Conclusion] The developed method is simple,accurate,rapid and convenient Lagotis brachystachya Maxim is rich in essential trace elements including Ca,Mg,Fe,Zn and Mn,which may be important for drug efficacy. |
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Keywords: | ICP-OES |
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