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| 不同凝胶组成对SSCP标记效果的比较分析 | |
| 引用本文： | 雷雪芹,陈宏,袁志发,雷初朝,孙维斌,李瑞彪. 不同凝胶组成对SSCP标记效果的比较分析[J]. 西北农业学报, 2003, 12(3): 9-11 |
| 作者姓名： | 雷雪芹 陈宏 袁志发 雷初朝 孙维斌 李瑞彪 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;河南科技大学动物科技学院,河南,洛阳,471003 2. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;徐州师范大学生物技术研究所,江苏,徐州,221009 3. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100 |
| 基金项目： | 国家863计划项目(2001AA243052);国家自然科学基金(30070551);杨凌示范区专项基金(99KG);中华农业科教基金(99-03-A-5);国家杨凌生物技术育种中心专项基金(1999-6). |
| 摘 要： | PCR—SSCP是一种新的分子遗传标记方法,但影响其实验效果的因素很多。本研究主要综合有关文献报道,应用3种不同组成的聚丙烯酰胺凝胶(丙烯酰胺/N,N′—亚甲基双丙烯酰胺分别为49:1,39:1,29:1)进行比较分析,其中以聚丙烯酰胺凝胶浓度为8％、丙烯酰胺/N,N′—亚甲基双丙烯酰胺比例为49:1、加5％甘油的组成的效果最佳,其带纹清晰,容易识别。 |
| 关 键 词： | PCR-SSCP 聚丙烯酰胺凝胶(PAGE) 甘油 |
| 文章编号： | 1004-1389(2003)03-0009-03 |
| 收稿时间： | 2002-12-02 |
| 修稿时间： | 2003-03-25 |
Comparison Analysis of Effects on PCR-SSCP Marker in Different Component of Polyacrylamide Gel |
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| LEI Xue-qin,CHEN Hong,YUAN Zhi-f,LEI Chu-zhao,SUN Wei-bin and LI Rui-biao. Comparison Analysis of Effects on PCR-SSCP Marker in Different Component of Polyacrylamide Gel[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2003, 12(3): 9-11 | |
| Authors: | LEI Xue-qin CHEN Hong YUAN Zhi-f LEI Chu-zhao SUN Wei-bin LI Rui-biao |
| Affiliation: | College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China;College of Animal Science and Technology, Henan Science and Technology University, Luoyang, Henan 471003, China;College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China;Institute of Biotechnology, Xuzhou Normal University, Xuzhou, Jiangsu 221009, China;College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China;College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China;College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China;College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China |
| Abstract: | PCR-SSCP is one new kind of methods of molecular markers. There are a lot of factors affecting the result of PCR-SSCP. In this experiment, 3 groups of different components of polyacrylamide gel were used to select the best one for further PCR-SSCP analysis. The results showed that best one is 8% polyacrylamide gel (Acr: Bis, 49:1) with 5% glycerol which can produce clear bands. |
| Keywords: | PCR-SSCP Polyacrylamide gel glycerol |
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